Electrospun PCL/gelatin composite nanofiber structures for effective guided bone regeneration membranes

被引:298
|
作者
Ren Ke [1 ]
Wang Yi [2 ]
Sun Tao [2 ]
Yue Wen [1 ]
Zhang Hongyu [2 ]
机构
[1] China Univ Geosci, Sch Engn & Technol, Beijing 100083, Peoples R China
[2] Tsinghua Univ, Dept Mech Engn, State Key Lab Tribol, Beijing 100084, Peoples R China
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 78卷
基金
中国国家自然科学基金;
关键词
Electrospinning; PCL/gelatin; Nanofiber; Guided bone regeneration membranes; TISSUE ENGINEERING APPLICATIONS; DRUG-DELIVERY; CROSS-LINKING; PCL; FABRICATION; SCAFFOLDS; GELATIN; BIOMATERIALS; PERFORMANCE; CHITOSAN;
D O I
10.1016/j.msec.2017.04.084
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Guided bone regeneration (GBR) membranes have been proved of great benefit for bone tissue engineering due to the improvement of cell attachment and proliferation. To develop GBR membranes with better biocompatibility and more proper degradation ability, here we fabricated polycaprolactone (PCL, polymer)/gelatin (protein) hybrid nanofibrous GBR membranes via electrospinning, followed by crosslinking with genipin. Acetic acid (HAc) was utilized to resolve the phase separation of PCL and gelatin, therefore homogeneous PCL/gelatin hybrid nanofibers with different ratios were successfully prepared. FTIR, XPS, TGA, DSC results proved that the proportion of PCL and gelatin in the as-spun nanofiber membranes could be simply adjusted by changing the weight ratio of PCL and gelatin in the spinning solution. SEM and AFM images demonstrated that all the nanofibers possessed uniform and smooth structures both in two dimension (2D) and three dimension (3D). The mechanical tests showed that these nanofibers exhibited appropriate tensile and strength properties, which were suitable for bone tissue engineering. CCK-8 and SEM images revealed that all the membranes were biocompatible to MC3T3-el cells. In addition, the in vitro osteogenesis characterizations, alizarin red in normal medium and osteogenesis medium, indicated that the nanofibers could promote bone formation. Therefore, all these results could suggest that our design of electrospun polymer/protein nanofiber membranes was effective for guided bone regeneration. (C) 2017 Published by Elsevier B.V.
引用
收藏
页码:324 / 332
页数:9
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