The Effects of Dexamethasone, Ascorbic Acid, and β-Glycerophosphate on Osteoblastic Differentiation by Regulating Estrogen Receptor and Osteopontin Expression
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作者:
Park, Jun-Beom
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Seoul Natl Univ, Dept Mol Med & Biopharmaceut Sci, Grad Sch Convergence Sci & Technol, Seoul, South KoreaSeoul Natl Univ, Dept Mol Med & Biopharmaceut Sci, Grad Sch Convergence Sci & Technol, Seoul, South Korea
Park, Jun-Beom
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机构:
[1] Seoul Natl Univ, Dept Mol Med & Biopharmaceut Sci, Grad Sch Convergence Sci & Technol, Seoul, South Korea
Background. Ascorbic acid (AA), beta-glycerophosphate (GP), and dexamethasone (DEX) are the compounds known to favor the expression of the osteoblastic phenotype in several bone cell systems. Materials and Methods. In this report, the combination effects of differentiation agents on osteoprecursor cells were evaluated. The effect on cell proliferation was determined by a cell viability test with morphologic analysis. Differentiation and mineralization were evaluated using an alkaline phosphatase activity test and alizarin red-S staining. Protein expressions related to bone formation, such as transforming growth factor-beta (TGF-beta), estrogen receptor-alpha (ER-alpha), and osteopontin (OPN) were evaluated by using a Western blot analysis. Results and Conclusion. AA and GP provided an inductive effect for differentiation of osteoprecusor cells, while short-term application of DEX seemed to lead to a dose-dependent increase of cellular differentiation. Long-term use of DEX seemed to reduce mineralization. These effects mayseem to be regulated by the expression of ER-alpha, OPN, and TGF-beta. Further studies related to this mechanism within the in vivo model may be necessary to ascertain greater detail. (C) 2012 Elsevier Inc. All rights reserved.
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Kyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Nagao, Satomi
Goto, Tetsuya
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Kagoshima Univ, Grad Sch Med & Dent Sci, Div Oral Anat & Histol, Kagoshima 8908544, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Goto, Tetsuya
Kataoka, Shinji
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Kyushu Dent Univ, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Kataoka, Shinji
Toyono, Takashi
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Kyushu Dent Univ, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Toyono, Takashi
Joujima, Takaaki
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Kyushu Dent Univ, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Joujima, Takaaki
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Egusa, Hiroshi
Yatani, Hirofumi
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Osaka Univ, Grad Sch Dent, Div Fixed Prosthodont, Suita, Osaka 5650871, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Yatani, Hirofumi
Kobayashi, Shigeru
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Kyushu Dent Univ, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan
Kobayashi, Shigeru
Maki, Kenshi
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Kyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, JapanKyushu Dent Univ, Div Dev Stomatognath Funct Sci, Kitakyushu, Fukuoka 8038580, Japan