Pectinolytic enzyme production by Bacillus species and their potential application on juice extraction

被引：38
|
作者：
Soares, MMCN [1 ]
Da Silva, R [1 ]
Carmona, EC [1 ]
Gomes, E [1 ]
机构：
[1] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, BR-15054000 Jd Nazareth, SP, Brazil
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2001年 / 17卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
juice extraction; pectinases; pectin lyases; polygalacturonases;
D O I：
10.1023/A:1016667930174
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Five Bacillus strains isolated from decaying vegetable material were cultivated on wheat bran and endo-polygalacturonases, exo-polygalacturonase and pectin lyase activities in the crude enzymatic solution obtained were determined. Highest activity was observed for all enzymes when fermentation was carried out at 28 degreesC, the highest activity values were obtained after 120 h of cultivation for exo-PG and after 48 h for endo-PG and PL. The use of the enzymatic solution for treatment of fruits and vegetable mash afforded a high juice extraction and a pulp with good pressing characteristics.
引用
收藏
页码：79 / 82
页数：4
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