Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis

被引：8
|
作者：
Higashide, Satoshi [1 ]
Cho, Otomi [1 ]
Matsuda, Yuko [1 ,2 ]
Ogishima, Daiki [2 ]
Kurakado, Sanae [1 ]
Sugita, Takashi [1 ]
机构：
[1] Meiji Pharmaceut Univ, Dept Microbiol, 2-522-1 Noshio, Tokyo 2048588, Japan
[2] Juntendo Nerima Hosp, Dept Obstet & Gynecol, 3-1-10 Takanodai, Tokyo 1778521, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 62卷 / 09期
关键词：
bacterial vaginosis; Gardnerella vaginalis; loop-mediated isothermal amplification; rapid detection; BACTERIAL VAGINOSIS; PRETERM DELIVERY; GRAM STAIN; DIAGNOSIS; ASSAY; LAMP; ALIGNMENT; ACCURATE; LOADS;
D O I：
10.1111/1348-0421.12641
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The aim of this study was to develop a method for the rapid detection of Gardnerella vaginalis, which is proposed to play a key role in the pathogenesis of bacterial vaginosis. Specific loop-mediated isothermal amplification (LAMP) primers were designed and used to detect target DNA within 45min under isothermal conditions. Comparative screening indicated that the LAMP assay is superior to PCR in terms of rapidity, and is equivalent in sensitivity and specificity. This LAMP assay can be used for rapid screening and detection of G. vaginalis in vaginal samples; the limit of detection is 10 fg DNA.
引用
收藏
页码：607 / 611
页数：5
相关论文
共 50 条
  • [21] Development of loop-mediated isothermal amplification for rapid detection of Entamoeba histolytica
    Windell L.Rivera
    Vanissa A.Ong
    Asian Pacific Journal of Tropical Medicine, 2013, (06) : 457 - 461
  • [22] Rapid and Sensitive Detection of Listeria monocytogenes by Loop-Mediated Isothermal Amplification
    Tang, Meng-Jun
    Zhou, Sheng
    Zhang, Xiao-Yan
    Pu, Jun-Hua
    Ge, Qing-Lian
    Tang, Xiu-Jun
    Gao, Yu-Shi
    CURRENT MICROBIOLOGY, 2011, 63 (06) : 511 - 516
  • [23] Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores
    Yan-Mei Qiao
    Yong-Chao Guo
    Xian-En Zhang
    Ya-Feng Zhou
    Zhi-Ping Zhang
    Hong-Ping Wei
    Rui-Fu Yang
    Dian-Bing Wang
    BIOTECHNOLOGY LETTERS, 2007, 29 (12) : 1939 - 1946
  • [24] Loop-mediated isothermal amplification for rapid detection of Chinese sacbrood virus
    Ma, Mingxiao
    Ma, Chen
    Li, Mng
    Wang, Shuquan
    Yang, Song
    Wang, Shude
    JOURNAL OF VIROLOGICAL METHODS, 2011, 176 (1-2) : 115 - 119
  • [25] Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae
    Jiahe Li
    F. Chris Minion
    Andrew C. Petersen
    Fei Jiang
    Sheng Yang
    Panpan Guo
    Jinxiang Li
    Wenxue Wu
    World Journal of Microbiology and Biotechnology, 2013, 29 : 607 - 616
  • [26] Loop-mediated isothermal amplification technology for rapid detection of plant viruses
    James, D.
    CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 2009, 31 (01): : 115 - 115
  • [27] Rapid and convenient detection of Weissella ceti with loop-mediated isothermal amplification
    Fang, Huahua
    Pan, Jiankun
    Wang, Yingfei
    Tao, Ye
    Zheng, Chaoli
    Wang, Minghao
    Qian, Dandan
    Sun, Yanling
    ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, 2024, 76 (04): : 327 - 334
  • [28] Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae
    Li, Jiahe
    Minion, F. Chris
    Petersen, Andrew C.
    Jiang, Fei
    Yang, Sheng
    Guo, Panpan
    Li, Jinxiang
    Wu, Wenxue
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2013, 29 (04): : 607 - 616
  • [29] Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
    Das, Amaresh
    Babiuk, Shawn
    McIntosh, Michael T.
    JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (05) : 1613 - 1620
  • [30] Rapid and Sensitive Detection of Listeria monocytogenes by Loop-Mediated Isothermal Amplification
    Meng-Jun Tang
    Sheng Zhou
    Xiao-Yan Zhang
    Jun-Hua Pu
    Qing-Lian Ge
    Xiu-Jun Tang
    Yu-Shi Gao
    Current Microbiology, 2011, 63
12345