Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis

被引：8
|
作者：
Higashide, Satoshi [1 ]
Cho, Otomi [1 ]
Matsuda, Yuko [1 ,2 ]
Ogishima, Daiki [2 ]
Kurakado, Sanae [1 ]
Sugita, Takashi [1 ]
机构：
[1] Meiji Pharmaceut Univ, Dept Microbiol, 2-522-1 Noshio, Tokyo 2048588, Japan
[2] Juntendo Nerima Hosp, Dept Obstet & Gynecol, 3-1-10 Takanodai, Tokyo 1778521, Japan
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 62卷 / 09期
关键词：
bacterial vaginosis; Gardnerella vaginalis; loop-mediated isothermal amplification; rapid detection; BACTERIAL VAGINOSIS; PRETERM DELIVERY; GRAM STAIN; DIAGNOSIS; ASSAY; LAMP; ALIGNMENT; ACCURATE; LOADS;
D O I：
10.1111/1348-0421.12641
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The aim of this study was to develop a method for the rapid detection of Gardnerella vaginalis, which is proposed to play a key role in the pathogenesis of bacterial vaginosis. Specific loop-mediated isothermal amplification (LAMP) primers were designed and used to detect target DNA within 45min under isothermal conditions. Comparative screening indicated that the LAMP assay is superior to PCR in terms of rapidity, and is equivalent in sensitivity and specificity. This LAMP assay can be used for rapid screening and detection of G. vaginalis in vaginal samples; the limit of detection is 10 fg DNA.
引用
收藏
页码：607 / 611
页数：5
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