APPLICATION OF ENZYMATIC PROCESS FOR BIODIESEL SYNTHESIS FROM VEGETABLE OIL WITH HIGH FATTY ACID CONTENT USING BUTANOL

Oil produced from spoiled rapeseed has high free fatty acid content. Such oil is not suitable for utilization for food production but could be used for biodiesel production by applying biotechnological methods. It was determined that the most effective biocatalyst for biodiesel synthesis from oil of 4 % acidity for oil transesterification with butanol was Lipozyme RM IM. Biodiesel, which met the standard requirements, was synthesized from rapeseed oil, applying the two-stage transesterification process, which optimal conditions were the following: for the first stage - 39.1 degrees C temperature, oil and butanol molar ratio - 1:4.5, 5.96 % of Lipozyme RM IM, 9.75 hours of reaction duration; for the second stage - the same as in the first stage temperature, oil and butanol molar ratio 1:1.5; 7.8 % of lipase, duration - 7.9 h. The physical and chemical properties of the made rapeseed oil butylesters were determined and compared with common biodiesel. According to the obtained results the principal technological design for enzymatic rapeseed oil butylesters production was designed.