High-resolution backscatter electron imaging of colloidal gold in LVSEM

被引：5
|
作者：
Erlandsen, S
Chen, Y
Frethem, C
Detry, J
Wells, C
机构：
[1] Univ Minnesota, Sch Med, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
[3] Honeywell Inc, Plymouth, MN 55447 USA
来源：
JOURNAL OF MICROSCOPY-OXFORD | 2003年 / 211卷
关键词：
BSE imaging; colloidal gold; contamination; detector threshold; ExB detector; low-voltage FESEM; YAG detector;
D O I：
10.1046/j.1365-2818.2003.01218.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
High-resolution backscatter electron (BSE) imaging of colloidal gold can be accomplished at low voltage using in-lens or below-the-lens FESEMs equipped with either Autrata-modified yttrium aluminium garnet (YAG) scintillators doped with cerium, or with BSE to secondary electron (SE) conversion plates. The threshold for BSE detection of colloidal gold was 1.8 keV for the YAG detector, and the BSE/SE conversion was sensitive down to 1 keV. Gold particles (6, 12 and 18 nm) have an atomic number of 79 and were clearly distinguished at 500 000x by materials contrast and easily discriminated from cell surfaces coated with platinum with an atomic number of 78. BSE imaging was relatively insensitive to charging, and build up of carbon contamination on the specimen was transparent to the higher energy BSE.
引用
收藏
页码：212 / 218
页数：7
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