A semiempirical model to control the production of a recombinant aldolase in high cell density cultures of Escherichia coli

被引：9
|
作者：
Ruiz, Jordi [1 ]
Gonzalez, Gloria [1 ]
de Mas, Caries [1 ]
Lopez-Santin, Josep [1 ]
机构：
[1] Univ Autonoma Barcelona, Dept Engn Quim, Escola Engn, Unitat Biocatalisi Aplicada Associada IIQA,CSIC, Bellaterra 08193, Spain
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2011年 / 55卷 / 02期
关键词：
Recombinant aldolase; Fed-batch; Escherichia coli; Mathematical modeling; Model-based control; Inducer-biomass ratio; FED-BATCH CULTURES; HIGH-LEVEL PRODUCTION; PROTEIN-PRODUCTION; ONLINE OPTIMIZATION; HETEROLOGOUS GENE; FEEDBACK-CONTROL; FOREIGN PROTEIN; KINETIC-MODEL; E; COLI; EXPRESSION;
D O I：
10.1016/j.bej.2011.03.001
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A semi-empirical mathematical model for recombinant rhamnulose 1-phosphate aldolase (RhuA) production in high cell density cultures of Escherichia coli has been developed. An unstructured and non-segregated approach allowed to identify the inducer-biomass ratio (I/X) as the key parameter influencing initial specific production rate and specific growth rate reduction after induction. Biomass, substrate and RhuA concentration profiles have been properly fitted in fed-batch cultures induced by an IPTG pulse at different I/X ratio ranging from 0 to 3 mu mol IPTG.g-1 dry cell weight. The model has been validated firstly as able to predict recombinant aldolase and biomass production. In addition, since glucose accumulation in the induced period, which is an indicator of activity reduction by proteolysis, can be anticipated, the model has been used for control purposes with excellent results. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：82 / 91
页数：10
相关论文
共 50 条
  • [31] Production of interferon-α in high cell density cultures of recombinant Escherichia coli and its single step purification from refolded inclusion body proteins
    K. R. Babu
    S. Swaminathan
    S. Marten
    N. Khanna
    U. Rinas
    Applied Microbiology and Biotechnology, 2000, 53 : 655 - 660
  • [32] Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain
    Park, SJ
    Georgiou, G
    Lee, SY
    BIOTECHNOLOGY PROGRESS, 1999, 15 (02) : 164 - 167
  • [33] Effects of glycerol on beta-lactamase production during high cell density cultivation of recombinant Escherichia coli
    Kwon, S
    Kim, S
    Kim, E
    BIOTECHNOLOGY PROGRESS, 1996, 12 (02) : 205 - 208
  • [34] Heat-induced production of human growth hormone by high cell density cultivation of recombinant Escherichia coli
    Tabandeh, F
    Shojaosadati, SA
    Zomorodipour, A
    Khodabandeh, M
    Sanati, MH
    Yakhchali, B
    BIOTECHNOLOGY LETTERS, 2004, 26 (03) : 245 - 250
  • [35] High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor
    Julia Glazyrina
    Eva-Maria Materne
    Thomas Dreher
    Dirk Storm
    Stefan Junne
    Thorsten Adams
    Gerhard Greller
    Peter Neubauer
    Microbial Cell Factories, 9
  • [36] Heat-Induced Production of Human Growth Hormone by High Cell Density Cultivation of Recombinant Escherichia Coli
    Fatemeh Tabandeh
    Sayed Abbas Shojaosadati
    Alireza Zomorodipour
    Mahvash Khodabandeh
    Mohammad H. Sanati
    Bagher Yakhchali
    Biotechnology Letters, 2004, 26 : 245 - 250
  • [37] Secretory production of recombinant protein by a high cell density culture of a protease negative mutant Escherichia coli strain
    Park, Si Jae
    Georgiou, George
    Lee, Sang Yup
    Biotechnology Progress, 15 (02): : 164 - 167
  • [38] On-line optimization and control of recombinant protein expression in high cell density Escherichia coli cultures using GFP-fusion monitoring.
    Chae, HJ
    DeLisa, MP
    Cha, HJ
    Li, JC
    Weigand, WA
    Valdes, JJ
    Rao, G
    Bentley, WE
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, 219 : U213 - U213
  • [39] The carbon-source on the efficiency of plasmid production in high density cultures of Escherichia coli
    Calado, C. R.
    Roxo-Rosa, M.
    Martins, J. G.
    NEW BIOTECHNOLOGY, 2009, 25 : S212 - S212
  • [40] PRODUCTION OF THERMALLY-INDUCED RECOMBINANT PROTEINS RELATIVE TO CELL BIOMASS IS INFLUENCED BY CELL-DENSITY IN ESCHERICHIA-COLI BATCH CULTURES
    CORCHERO, JL
    VILA, P
    CUBARSI, R
    VILLAVERDE, A
    BIOTECHNOLOGY LETTERS, 1994, 16 (08) : 777 - 782
12345