Selective differential fingerprinting - A method for identifying differentially expressed genes in a family between two samples

被引：1
|
作者：
Weber, KL [1 ]
Bolander, ME [1 ]
Sarkar, G [1 ]
机构：
[1] Mayo Clin, Rochester, MN 55905 USA
来源：
MOLECULAR BIOTECHNOLOGY | 1998年 / 10卷 / 01期
关键词：
gene family; differential display PCR; expression; TGF-beta; nested PCR;
D O I：
10.1007/BF02745864
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method termed selective differential fingerprinting (SDF) has been developed that enables one to investigate the level of expression for a family of genes between two samples. SDF produces a fingerprint (on a sequencing gel) on reverse transcription polymerase chain reaction (RT-PCR) of a sample with degenerate primers designed from conserved regions of a family of genes. By comparing fingerprints obtained after SDF with primers representing the transforming growth factor-beta (TGF beta) family of growth factors between a low-grade and a high-grade tumor from the same patient, a TGF beta family member known as osteogenic protein 1 (OP-1) or bone morphogenic protein 7 (BMP-7) was found to be greatly overexpressed in the high-grade tumor compared to the low-grade one. SDF also has the potential to identify novel genes. SDF offers a general way to identify differentially expressed genes for a family between two given samples.
引用
收藏
页码：77 / 81
页数：5
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