This paper discussed the transboundary eco-security' issues, their distribution patterns, characteristics and driving forces, and regulation system in the Longitudinal Range-Gorge Region.(LRGR). Major transboundary eco-security issues, except for sand-dust storm, can be found along the terrestrial border regions of China in the LRGR. The direct driving forces included constructions of cascade hydropower dams, highways and international navigation. development, slope land cultivation and mineral exploration. Transboundary eco-security issues affect the neighboring areas in the terrestrial region, and the downstream countries along the international watercourses. The ecological changes in the neighboring countries had effects on the domestic side in the LRGR, such as species invasion. The integrated measures should be taken into consideration, including the construction of border eco-protection projects and transboundary nature reserves, ecological threshold set-up, species invasion control, the establishment of eco-security monitoring platform, the constituting of ecological compensation mechanism and laws (rules) and institutions, and capacity building. The most important tasks to deal with the transboundary issues in the LRGR are to form internationally participative cooperation mechanism, establish fund for transboundary eco-security, implement ecological compensation strategy, and develop information exchange platform and warning system.