8-oxoguanine induces intramolecular DNA damage but free 8-oxoguanine protects intermolecular DNA from oxidative stress
被引:34
|
作者:
Kim, JE
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Pharmacol, Seoul 110799, South KoreaSeoul Natl Univ, Dept Pharmacol, Seoul 110799, South Korea
Kim, JE
[1
]
Choi, S
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Pharmacol, Seoul 110799, South KoreaSeoul Natl Univ, Dept Pharmacol, Seoul 110799, South Korea
Choi, S
[1
]
Yoo, JA
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Pharmacol, Seoul 110799, South KoreaSeoul Natl Univ, Dept Pharmacol, Seoul 110799, South Korea
Yoo, JA
[1
]
Chung, MH
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Dept Pharmacol, Seoul 110799, South KoreaSeoul Natl Univ, Dept Pharmacol, Seoul 110799, South Korea
Chung, MH
[1
]
机构:
[1] Seoul Natl Univ, Dept Pharmacol, Seoul 110799, South Korea
来源:
FEBS LETTERS
|
2004年
/
556卷
/
1-3期
关键词:
oxidative stress;
8-oxoguanine;
electron transfer;
D O I:
10.1016/S0014-5793(03)01385-1
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
7,8-Dihydro-8-oxoguanine (8-oxoguanine; 8-oxo-G), one of the major oxidative DNA adducts, is highly susceptible to further oxidation by radicals. We confirmed the higher reactivity of 8-oxo-G toward reactive oxygen (singlet oxygen and hydroxyl radical) or nitrogen (peroxynitrite) species as compared to unmodified base. In this study, we raised the question about the effect of this high reactivity toward radicals on intramolecular and intermolecular DNA damage. We found that the amount of intact nucleoside in oligodeoxynucleotide containing 8-oxo-G decreased more by various radicals at higher levels of 8-oxo-G incorporation, and that the oligodeoxynucleotide damage and plasmid cleavage by hydroxyl radical were inhibited in the presence of 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dG). We conclude that 8-oxo-G within DNA induces intramolecular DNA base damage, but that free 8-oxo-G protects intermolecular DNA from oxidative stress. These results suggest that 8-oxo-G within DNA must be rapidly released to protect DNA from overall oxidative damage. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.