EPC-K1 attenuates peroxynitrite-induced apoptosis in cerebellar granule cells

被引：0
|
作者：
Wei, TT
Chen, C
Zhao, BL
Xin, WJ [1 ]
Mori, A
机构：
[1] Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China
[2] Okayama Univ, Sch Med, Dept Neurosci, Inst Mol & Cellular Med, Okayama 700, Japan
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1998年 / 46卷 / 01期
关键词：
cerebellar granule cells; apoptosis; peroxynitrite; antioxidants; EPC-K1;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Apoptosis induced by peroxynitrite in cultured cerebellar granule cells was confirmed morphologically by chromatin condensation and biochemically by DNA laddering. A 30 min exposure to peroxynitrite (10 mu M) initiated oxidative stress, which caused the formation of thiobarbituric acid-reactive substances (TBARS) and the alteration of cell membrane fluidity. Peroxynitrite treatment also caused ATP decrease and thus activated the apoptotic program. Pre-treating cells with antioxidant EPC-K1 (L-ascorbic acid 2-[3,4-dihydro-2,5,7,8-tetramethyl-2-(4,8, 12-trimethyltridecyl)-2H-1-benzopyran-6-yl-hydrogen phosphate] potassium salt), a new water-soluble derivative of vitamin C and vitamin E, attenuated oxidative injury and prevents cells from apoptosis. The results suggest that EPC-KI might be used as a potential therapeutic agent for diseases associated with NO/ONOO--mediated neuronal injury.
引用
收藏
页码：89 / 97
页数：9
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