Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

被引:24
|
作者
Teixeira, D. G. [1 ,2 ]
Monteiro, G. R. G. [2 ]
Martins, D. R. A. [1 ,3 ]
Fernandes, M. Z. [4 ]
Macedo-Silva, V. [1 ]
Ansaldi, M. [5 ]
Nascimento, P. R. P. [1 ]
Kurtz, M. A. [6 ]
Streit, J. A. [6 ,7 ]
Ximenes, M. F. F. M. [5 ]
Pearson, R. D. [8 ]
Miles, A. [9 ]
Blackwell, J. M. [10 ]
Wilson, M. E. [6 ,7 ,11 ,12 ]
Kitchen, A. [13 ]
Donelson, J. E. [2 ,14 ]
Lima, J. P. M. S. [1 ,2 ]
Jeronimo, S. M. B. [1 ,2 ,15 ]
机构
[1] Univ Fed Rio Grande do Norte, Biosci Ctr, Dept Biochem, Natal, RN, Brazil
[2] Inst Trop Med Rio Grande Norte, Natal, RN, Brazil
[3] Univ Fed Rio Grande do Norte, Biosci Ctr, Dept Cellular Biol & Genet, Natal, RN, Brazil
[4] Univ Fed Rio Grande do Norte, Dept Internal Med, Natal, RN, Brazil
[5] Univ Fed Rio Grande do Norte, Dept Microbiol & Parasitol, Natal, RN, Brazil
[6] Vet Affairs Med Ctr, Iowa City, IA 52242 USA
[7] Univ Iowa, Dept Internal Med, Iowa City, IA 52242 USA
[8] Univ Virginia, Dept Internal Med, Div Infect Dis, Charlottesville, VA USA
[9] Univ Oxford, Wellcome Trust Ctr Human Genet, Roosevelt Dr, Oxford, England
[10] Univ Western Australia, Telethon Inst Child Hlth, Perth, WA, Australia
[11] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA
[12] Univ Iowa, Dept Epidemiol, Iowa City, IA USA
[13] Univ Iowa, Dept Anthropol, Iowa City, IA USA
[14] Univ Iowa, Dept Biochem, Iowa City, IA 52242 USA
[15] Natl Inst Sci & Technol Trop Dis, Natal, RN, Brazil
基金
美国国家卫生研究院;
关键词
Leishmania infantum; Intracellular parasite; Evolution; Parasite population structure; Copy number variation; Brazil; VISCERAL LEISHMANIASIS; POPULATION-GENETICS; KALA-AZAR; INFECTION; DONOVANI; EPIDEMIOLOGY; GENERATION; ANEUPLOIDY; DIVERSITY; INSIGHTS;
D O I
10.1016/j.ijpara.2017.04.004
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The genomic sequences of 20 Leishmania infantum isolates collected in northeastern Brazil were compared with each other and with the available genomic sequences of 29 L. infantum/donovani isolates from Nepal and Turkey. The Brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. Two isolates were from the blood and bone marrow of the same visceral leishmaniasis patient. All 20 genomic sequences display 99.95% identity with each other and slightly less identity with a reference L. infantum genome from a Spanish isolate. Despite the high identity, analysis of individual differences among the 32 million base pair genomes showed sufficient variation to allow the isolates to be clustered based on the primary sequence. A major source of variation detected was in chromosome somy, with only four of the 36 chromosomes being predominantly disomic in all 49 isolates examined. In contrast, chromosome 31 was predominantly tetrasomic/pentasomic, consistent with its regions of synteny on two different disomic chromosomes of Trypanosoma brucei. In the Brazilian isolates, evidence for recombination was detected in 27 of the 36 chromosomes. Clustering analyses suggested two populations, in which two of the five older isolates from the 1990s clustered with a majority of recent isolates. Overall the analyses do not suggest individual sequence variants account for differences in clinical outcome or adaptation to different hosts. For the first known time, DNA of isolates from asymptomatic subjects were sequenced. Of interest, these displayed lower diversity than isolates from symptomatic subjects, an observation that deserves further investigation with additional isolates from asymptomatic subjects. (C) 2017 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:655 / 665
页数:11
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