Effects of high-energy-pulse-electron beam radiation on biomacromolecules

被引：18

作者：

Zhu Hong ^{[1
]}

Xu JingZao ^{[1
]}

Li ShiQiang ^{[1
]}

Sun XiaoYu ^{[1
]}

Yao SiDe ^{[1
,2
]}

Wang ShiLong ^{[1
]}

机构：

[1] Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

来源：

SCIENCE IN CHINA SERIES B-CHEMISTRY | 2008年 / 51卷 / 01期

基金：

中国国家自然科学基金;

关键词：

high-energy-pulse-electron beam; biomacromolecule; radiation damage;

D O I：

10.1007/s11426-008-0017-4

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by Co-60 gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.

引用

页码：86 / 91

页数：6

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