A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection

被引：20
|
作者：
Zhu, Liangliang [1 ]
Hao, Hongshun [1 ,2 ]
Ding, Chao [1 ]
Gan, Hanwei [1 ]
Jiang, Shuting [1 ]
Zhang, Gongliang [2 ]
Bi, Jingran [2 ]
Yan, Shuang [1 ]
Hou, Hongman [2 ]
机构：
[1] Dalian Polytech Univ, Dept Inorgan Nonmetall Mat Engn, Dalian 116034, Peoples R China
[2] Dalian Polytech Univ, Sch Food Sci & Technol, Liaoning Key Lab Aquat Proc Qual & Safety, Dalian 116034, Peoples R China
来源：
FOODS | 2021年 / 10卷 / 12期
关键词：
photoelectrochemical detection; aptamer; Listeria monocytogenes; quantum dots; exonuclease I;
D O I：
10.3390/foods10122896
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 x 10(1)-1.3 x 10(7) CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes.
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收藏
页数：19
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