A Novel Photoelectrochemical Aptamer Sensor Based on CdTe Quantum Dots Enhancement and Exonuclease I-Assisted Signal Amplification for Listeria monocytogenes Detection

被引：20
|
作者：
Zhu, Liangliang [1 ]
Hao, Hongshun [1 ,2 ]
Ding, Chao [1 ]
Gan, Hanwei [1 ]
Jiang, Shuting [1 ]
Zhang, Gongliang [2 ]
Bi, Jingran [2 ]
Yan, Shuang [1 ]
Hou, Hongman [2 ]
机构：
[1] Dalian Polytech Univ, Dept Inorgan Nonmetall Mat Engn, Dalian 116034, Peoples R China
[2] Dalian Polytech Univ, Sch Food Sci & Technol, Liaoning Key Lab Aquat Proc Qual & Safety, Dalian 116034, Peoples R China
来源：
FOODS | 2021年 / 10卷 / 12期
关键词：
photoelectrochemical detection; aptamer; Listeria monocytogenes; quantum dots; exonuclease I;
D O I：
10.3390/foods10122896
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
To achieve the rapid detection of Listeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 x 10(1)-1.3 x 10(7) CFU/mL. The construction strategy provides a new way to detect Listeria monocytogenes.
引用
收藏
页数：19
相关论文
共 50 条
  • [1] Sensitive and Facile Detection of Vitamin D Based on Fluorescent Labeled Aptamer Probe and Exonuclease I-Assisted Signal Amplification
    Xu, Xinqiu
    Jia, Chaofan
    Zhang, Fengjiao
    Li, Hao
    Gong, Weilei
    Wang, Changqin
    Wei, Yin
    JOURNAL OF FLUORESCENCE, 2025,
  • [2] Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin
    Li, Jingwen
    Liu, Yongming
    Lin, Hao
    Chen, Yan
    Liu, Zhenbo
    Zhuang, Xuming
    Tian, Chunyuan
    Fu, Xiuli
    Chen, Lingxin
    FOOD CHEMISTRY, 2021, 347
  • [3] Photoelectrochemical aptasensor for kanamycin determination based on exonuclease I-assisted target signal amplification and photoexcited electron transfer strategy
    Wen, Ruiting
    Zhang, Yihang
    Zhou, Chunhong
    Huang, Mingjuan
    Tian, Jiuying
    Lu, Jusheng
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2022, 906
  • [4] Exonuclease I-Assisted General Strategy to Convert Aptamer-Based Electrochemical Biosensors from "Signal-Off" to "Signal-On"
    Gao, Xiaoyi
    Qi, Lin
    Liu, Kun
    Meng, Chenchen
    Li, Yunchao
    Yu, Hua-Zhong
    ANALYTICAL CHEMISTRY, 2020, 92 (09) : 6229 - 6234
  • [5] A novel and sensitive electrochemical aptasensor for sulfadimethoxine detection based on the triple helix/exonuclease I-assisted double-amplification strategy
    Du, Meijuan
    Cheng, Xin
    Chen, Qian
    Xu, Xueqin
    ANALYTICAL METHODS, 2024, 16 (11) : 1570 - 1578
  • [6] Homogeneous electrochemical aptasensor for mucin 1 detection based on exonuclease I-assisted target recycling amplification strategy
    Lin, Cuiying
    Zheng, Huixia
    Huang, Yaying
    Chen, Zhuling
    Luo, Fang
    Wang, Jian
    Guo, Longhua
    Qiu, Bin
    Lin, Zhenyu
    Yang, Huanghao
    BIOSENSORS & BIOELECTRONICS, 2018, 117 : 474 - 479
  • [7] Highly Sensitive β-Lactoglobulin Fluorescent Aptamer Biosensors Based on Tungsten Disulfide Nanosheets and DNase I-Assisted Signal Amplification
    Wang, Yuying
    Chen, Sisi
    Chen, Wanmei
    Wang, Jingjing
    Li, Kun
    Hong, Chengyi
    Zhang, Kailong
    Chen, Quansheng
    MOLECULES, 2023, 28 (08):
  • [8] Dual-target electrochemical DNA sensor for detection of Pb2+ and Hg2+ simultaneously by exonuclease I-assisted recycling signal amplification
    Wang, Yue
    Zhai, Hongguo
    Yin, Jiaqi
    Guo, Qi
    Zhang, Yuhao
    Yang, Qingqing
    Li, Falan
    Sun, Xia
    Guo, Yemin
    Zhang, Yanyan
    MICROCHIMICA ACTA, 2022, 189 (12)
  • [9] Signal-on Photoelectrochemical bioassay for DNA based on CdTe quantum dots by endonuclease-aided cycling amplification strategy
    Zhao, Yang
    Li, Zhi
    Kuang, Qian
    Jie, Guifen
    JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2018, 812 : 68 - 73
  • [10] Dual-signal aptamer sensor based on polydopamine-gold nanoparticles and exonuclease I for ultrasensitive malathion detection
    Xu, Guoli
    Hou, Jingzhou
    Zhao, Yanan
    Bao, Jing
    Yang, Mei
    Fa, Huanbao
    Yang, Yixia
    Li, Li
    Huo, Danqun
    Hou, Changjun
    SENSORS AND ACTUATORS B-CHEMICAL, 2019, 287 : 428 - 436
12345