Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate Alexandrium tamarense

被引：14
|
作者：
Cho, Yuko [1 ]
Ogawa, Motoo [2 ]
Hirota, Mayumi [3 ]
Oshima, Yasukatsu [2 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Tohoku Univ, Grad Sch Life Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[3] Tohoku Univ, Fac Agr, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
HARMFUL ALGAE | 2011年 / 10卷 / 03期
关键词：
Mitomycin C; Colchicine; Alexandrium tamarense; Paralytic shellfish toxins; PARALYTIC SHELLFISH; GYMNODINIUM-CATENATUM; SAXITOXIN; DINOPHYCEAE; TOXICITY; GROWTH; TEMPERATURE; BIVALVES; DYNAMICS; DIVISION;
D O I：
10.1016/j.hal.2010.10.003
中图分类号：
Q17 [水生生物学];
学科分类号：
071004 ;
摘要：
Paralytic shellfish toxin (PST) production and cell proliferation in the dinoflagellate Alexandrium tamarense were investigated under the influence of two metabolic inhibitors (mitomycin C and colchicine) that have different mechanisms of action. Intracellular PST levels in cells treated with 2 mu M mitomycin C increased gradually, reaching a maximum of 176 fmol/cell (a 6-fold increase). High concentrations of colchicine prolonged G(1) phase in A. tamarense cells, even though colchicine arrests several other eukaryotic cell types in M phase. The cells in prolonged G(1) phase under the influence of colchicine were apparently unable to produce PST. Cell proliferation and toxin production recovered after removal of colchicine; in contrast, the effect of mitomycin C was irreversible. While the timing of toxin production within the cell cycle was not conclusively determined, A. tamarense cells in S phase were able to produce PST. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：235 / 244
页数：10
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