Seohaeicola zhoushanensis sp nov., isolated from seawater

A novel, Gram-stain-negative, motile, facultatively anaerobic bacterium, designated strain NF48(T), was isolated from surface seawater around Zhoushan Islands. Cells were rod-shaped (1.1-3.9 x 0.5-0.9 mu m). Strain NF48(T) was able to grow at 10-40 degrees C (optimum 30 degrees C), at pH 5.5-9.0 (optimum 6.5-8.0) and with 0.5-7.0%(w/v) NaCl (optimum 2.0 %). The genomic DNA G+C content was 65.5 mol%. Chemotaxonomic analysis showed that the main isoprenoid quinone was Q-10 and the major fatty acids were C-18:1 omega 7c and C-16:0. The major polar lipids of strain NF48(T) were phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NF48(T) belonged to the genus Seohaeicola with similarity values of 95.5-97.2% to members of this genus, and was most closely related to Seohaeicola nanhaiensis SS011A0-7#2-2(T) (97.2 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, strain NF48(T) is suggested to represent a novel species of the genus Seohaeicola, for which the name Seohaeicola zhoushanensis sp. nov. is proposed. The type strain is NF48(T) (= MCCC 1K01157(T) = KCTC 42650(T)).