Co-delivery of curcumin and Bcl-2 siRNA by PAMAM dendrimers for enhancement of the therapeutic efficacy in HeLa cancer cells

被引：99
|
作者：
Ghaffari, Maryam [1 ,2 ,3 ]
Dehghan, Gholamreza [2 ]
Baradaran, Behzad [4 ]
Zarebkohan, Amir [5 ]
Mansoori, Behzad [4 ]
Soleymani, Jafar [6 ]
Dolatabadi, Jafar Ezzati Nazhad [7 ]
Hamblin, Michael R. [8 ,9 ,10 ]
机构：
[1] Tabriz Univ Med Sci, Res Ctr Pharmaceut Nanotechnol, Tabriz, Iran
[2] Univ Tabriz, Fac Nat Sci, Dept Biol, Tabriz, Iran
[3] Tabriz Univ Med Sci, Student Res Comm, Tabriz, Iran
[4] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran
[5] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Med Nanotechnol, Tabriz, Iran
[6] Tabriz Univ Med Sci, Pharmaceut Anal Res Ctr, Tabriz, Iran
[7] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran
[8] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA
[9] Harvard Med Sch, Dept Dermatol, Boston, MA 02115 USA
[10] Univ Johannesburg, Fac Hlth Sci, Laser Res Ctr, ZA-2028 Doornfontein, South Africa
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2020年 / 188卷
关键词：
Curcumin; Bcl-2; siRNA; PAMAM dendrimer; Co-delivery; HeLa cancer cells; Nanoparticles; DENDROSOMAL CURCUMIN; ANTICANCER ACTIVITY; INDUCE APOPTOSIS; BREAST-CANCER; IN-VITRO; NANOPARTICLES; DRUG; DOXORUBICIN; CISPLATIN; PATHWAYS;
D O I：
10.1016/j.colsurfb.2019.110762
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Co-delivery of therapeutic agents and small interfering RNA (siRNA) can be achieved by a suitable nanovehicle. In this work, the solubility and bioavailability of curcumin (Cur) were enhanced by entrapment in a poly-amidoamine (PAMAM) dendrimer, and a polyplex was formed by grafting Bcl-2 siRNA onto the surface amine groups to produce PAMAM-Cur/Bcl-2 siRNA nanoparticles (NPs). The synthesized polyplex NPs had a particle size of similar to 180 nm, and high Cur loading content of similar to 82 wt%. Moreover, the PAMAM-Cur/Bcl-2 siRNA NPs showed more effective cellular uptake, and higher inhibition of tumor cell proliferation compared to PAMAM-Cur nanoformulation and free Cur, due to the combined effect of co-delivery of Cur and Bcl-2 siRNA. The newly described PAMAM-Cur/Bcl-2 siRNA polyplex NPs could be a promising co-delivery nanovehicle.
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页数：11
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