Creation of a High-Yield AAV Vector Production Platform in Suspension HEK293T Cells Using a Design of Experiment Approach

被引:0
|
作者
Zhao, Huiren [1 ]
Lee, Ki Jeong [1 ]
Lin, Yun [1 ]
Wolfe, Thomas [1 ]
Sheng, Jackie [1 ]
Plewa, Cherylene [1 ]
Wang, Songli [1 ]
Meisen, W. Hans [1 ]
机构
[1] Amgen Inc, Genome Anal Unit, San Francisco, CA USA
关键词
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
441
引用
收藏
页码:196 / 197
页数:2
相关论文
共 44 条
  • [21] Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology
    C. Madeira
    S. C. Ribeiro
    M. Z. Turk
    J. M. S. Cabral
    Biotechnology Letters, 2010, 32 : 1393 - 1399
  • [22] Optimization of gene delivery to HEK293T cells by microporation using a central composite design methodology
    Madeira, C.
    Ribeiro, S. C.
    Turk, M. Z.
    Cabral, J. M. S.
    BIOTECHNOLOGY LETTERS, 2010, 32 (10) : 1393 - 1399
  • [23] High-yield extracellular vesicle production from HEK293T cells encapsulated in 3D auxetic scaffolds with cyclic mechanical stimulation for effective drug carrier systems
    Chen, Yi-Wen
    Lin, Yen-Hong
    Ho, Chia-Che
    Chen, Cheng-Yu
    Yu, Min-Hua
    Lee, Alvin Kai-Xing
    Chiu, Shao-Chih
    Cho, Der-Yang
    Shie, Ming-You
    BIOFABRICATION, 2024, 16 (04)
  • [24] Investigating dynamics of lentiviral vector secretion from HEK293T producer cells using a fractionated perfusion system
    Timmins, Lauren M.
    Erickson, Patrick
    Parekkadan, Biju
    BIOTECHNOLOGY JOURNAL, 2024, 19 (01)
  • [25] Development and Scale-Up of Bioreactor Process for Transient Lentivirus Production Using a Suspension-Adapted HEK293T Clone
    Patel, Sonal
    Fong, Eva
    George, Henry
    MOLECULAR THERAPY, 2020, 28 (04) : 433 - 434
  • [26] Reconfiguration of AAV Rep-Cap coding sequences significantly increases viral vector yield and enables inducible AAV production in HEK293 cells
    Bray, A.
    Janer, A. Barber
    Branciaroli, C.
    Su, W.
    Liu, Q.
    Payne, T.
    Cawood, R.
    HUMAN GENE THERAPY, 2018, 29 (12) : A154 - A154
  • [27] Automated HEK293 suspension cell adaptation to new media and use of new DoE approach to optimize AAV production in suspension utilizing the ambr®15 platform
    Robin, C.
    Enga, M.
    Menard, L.
    Pennors, M.
    Blouin, V.
    Adjali, O.
    Houyoux, V.
    Zuban, R.
    Vicard, Q.
    Ayuso, E.
    HUMAN GENE THERAPY, 2019, 30 (11) : A32 - A32
  • [28] Development of a Serum-Free Suspension HEK293 Cell Line for AAV Production with High Yield and Low Encapsidated DNA Impurities
    Yang, Hairui
    MOLECULAR THERAPY, 2024, 32 (04) : 511 - 511
  • [29] Development of an Assay to Perform High-Throughput Drug Screen in HEK293 Cells to Improve AAV Vector Production
    Panthi, Santosh
    Baer, Alan
    Bhattarai, Nirjal
    MOLECULAR THERAPY, 2024, 32 (04) : 711 - 711
  • [30] Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region
    Bae, Dahae Hailey
    Marino, Michael
    Iaffaldano, Brian
    Fenstermaker, Sydney
    Afione, Sandra
    Argaw, Takele
    McCright, Jacob
    Kwilas, Anna
    Chiorini, John A.
    Timmons, Andrew E.
    Reiser, Jakob
    MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT, 2020, 18 : 631 - 638