Applications of Mass Spectrometry to Lipids and Membranes

被引:149
|
作者
Harkewicz, Richard [1 ]
Dennis, Edward A.
机构
[1] Univ Calif San Diego, Sch Med, Dept Chem & Biochem, La Jolla, CA 92093 USA
来源
关键词
CLASS; DXMS; imaging mass spectrometry; lipidomics; novel lipids; shotgun; ELECTROSPRAY-IONIZATION; DEUTERIUM-EXCHANGE; PHOSPHOLIPASE A(2); PROTEIN-STRUCTURE; TISSUE-SECTIONS; CRUDE EXTRACTS; ADRENIC ACID; LIPIDOMICS; IDENTIFICATION; CHROMATOGRAPHY;
D O I
10.1146/annurev-biochem-060409-092612
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipidomics, a major part of metabolomics, constitutes the detailed analysis and global characterization, both spatial and temporal, of the structure and function of lipids (the lipidome) within a living system. As with proteomics, mass spectrometry has earned a central analytical role in lipidomics, and this role will continue to grow with technological developments. Currently, there exist two mass spectrometry based lipidomics approaches, one based on a division of lipids into categories and classes prior to analysis, the "comprehensive lipidomics analysis by separation simplification" (CLASS), and the other in which all lipid species are analyzed together without prior separation, shotgun. In exploring the lipidome of various living systems, novel lipids are being discovered, and mass spectrometry is helping characterize their chemical structure. Deuterium exchange mass spectrometry (DXMS) is being used to investigate the association of lipids and membranes with proteins and enzymes, and imaging mass spectrometry (IMS) is being applied to the in situ analysis of lipids in tissues.
引用
收藏
页码:301 / 325
页数:25
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