Pilot-scale demonstration of a novel process integrating Partial Nitritation with simultaneous Anammox, Denitrification and Sludge Fermentation (PN plus ADSF) for nitrogen removal and sludge reduction

Anammox process is a cost-effective solution for nitrogen removal, whereas unsatisfactory effluent with nitrate accumulation is usually achieved in treating domestic sewage, owning to the unwanted prevalence of nitrite-oxidizing bacteria (NOB) and the intrinsic nitrate production by anammox bacteria. Herein, a pilot-scale system integrating Partial Nitritation and simultaneous Anammox, Denitrification and Sludge Fermentation (PN + ADSF) process was developed to treat real municipal wastewater. In this process, PN was accomplished in a sequencing batch reactor (SBR) using the strategy of intermittent hydroxylamine addition, while ADSF coupling anammox and heterotrophic denitrification was conducted in an up-flow anaerobic sludge blanket reactor (UASB) to further remove nitrogen. The pilotscale system achieved total inorganic nitrogen (TIN) concentrations of 10.0 mg N/L in effluent and sludge reduction efficiency of 42.3% simultaneously. The characterization on microbial communities revealed that Candidatus Kuenenia and Thauera were the dominant functional bacteria for anammox and denitrification, respectively. Supported by the slow-release carbon sources from sludge fermentation, heterotrophic denitrification contributed to about 28% of nitrogen removed from the UASB, while anammox played a more important role in nitrogen removal. The pilot-scale demonstration confirmed that the PN + ADSF process is technically feasible for enhanced nitrogen removal and sludge reduction.