Comparison of the properties of collagen-chitosan scaffolds after γ-ray irradiation and carbodiimide cross-linking

被引:20
|
作者
Chen, Zihao [1 ]
Du, Tianming [1 ]
Tang, Xiangyu [1 ]
Liu, Changjun [1 ]
Li, Ruixin [1 ]
Xu, Cheng [1 ]
Tian, Feng [1 ]
Du, Zhenjie [1 ]
Wu, Jimin [1 ]
机构
[1] Acad Mil Med Sci, Inst Med Equipment, Dept Biomed Mat, Tianjin, Peoples R China
关键词
Biomaterial; porous materials; cross-linking; gamma-ray irradiation; carbodiimide; mechanical property; HYDROGELS;
D O I
10.1080/09205063.2016.1169478
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The property of collagen-chitosan porous scaffold varies according to cross-linking density and scaffold composition. This study was designed to compare the properties of collagen-chitosan porous scaffolds cross-linked with gamma-irradiation and carbodiimide (CAR) for the first time. Eleven sets of collagen-chitosan scaffolds containing different concentrations of chitosan at a 5% increasing gradient were fabricated. Fourier transform infrared spectroscopy was performed to confirm the success of cross-linking in the scaffolds. The scaffold morphology was evaluated under scanning electron microscope (SEM). SEM revealed that chitosan was an indispensable material for the fabrication of gamma-ray irradiation scaffold. The microstructure of gamma-ray irradiation scaffold was less stable than those of alternative scaffolds. Based upon swelling ratio, porosity factor, and collagenase degradation, gamma-ray irradiation scaffold was less stable than CAR and 25% proportion of chitosan scaffolds. Mechanical property determines the orientation in gamma-irradiation and CAR scaffold. In vitro degradation test indicated that gamma-irradiation and CAR cross-linking can elevate the scaffold biocompatibility. Compared with gamma-ray irradiation, CAR cross-linked scaffold containing 25% chitosan can more significantly enhance the bio-stability and biocompatibility of collagen-chitosan scaffolds. CAR cross-linked scaffold may be the best choice for future tissue engineering.
引用
收藏
页码:937 / 953
页数:17
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