RAPID DETECTION OF APPLE STEM GROOVING VIRUS BY REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

被引：2
|
作者：
Zhao, L. [1 ]
Feng, C. -H. [2 ]
Li, B. -Q. [2 ]
Hao, X. -A. [1 ]
Liu, H. [1 ]
Wu, Y. -F. [1 ]
Wang, Q. -C. [2 ]
机构：
[1] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Peoples R China
[2] Northwest A&F Univ, Coll Hort,State Key Lab Crop Stress Biol Arid Are, Minist Agr,Key Lab Plant Protect Resources & Pest, Key Lab Crop Pest Integrated Pest Management Crop, Yangling 712100, Peoples R China
来源：
JOURNAL OF PLANT PATHOLOGY | 2014年 / 96卷 / 02期
关键词：
Apple stem grooving virus; LAMP; detection; diagnosis; FRUIT; RNA; DNA;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Apple stem grooving virus (ASGV) is one of the relevant apple viruses worldwide. In this study, a simple, sensitive one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of ASGV, using primers designed in the coat protein gene. The RT-LAMP assay identified 53 infected apple leaf samples while RT-PCR identified only 49 infected samples. To the best of our knowledge, this is the first report on detection of ASGV by RT-LAMP.
引用
收藏
页码：407 / 409
页数：3
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