RAPID DETECTION OF APPLE STEM GROOVING VIRUS BY REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION

被引：2

作者：

Zhao, L. ^{[1
]}

Feng, C. -H. ^{[2
]}

Li, B. -Q. ^{[2
]}

Hao, X. -A. ^{[1
]}

Liu, H. ^{[1
]}

Wu, Y. -F. ^{[1
]}

Wang, Q. -C. ^{[2
]}

机构：

[1] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Peoples R China

[2] Northwest A&F Univ, Coll Hort,State Key Lab Crop Stress Biol Arid Are, Minist Agr,Key Lab Plant Protect Resources & Pest, Key Lab Crop Pest Integrated Pest Management Crop, Yangling 712100, Peoples R China

来源：

JOURNAL OF PLANT PATHOLOGY | 2014年 / 96卷 / 02期

关键词：

Apple stem grooving virus; LAMP; detection; diagnosis; FRUIT; RNA; DNA;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Apple stem grooving virus (ASGV) is one of the relevant apple viruses worldwide. In this study, a simple, sensitive one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid detection of ASGV, using primers designed in the coat protein gene. The RT-LAMP assay identified 53 infected apple leaf samples while RT-PCR identified only 49 infected samples. To the best of our knowledge, this is the first report on detection of ASGV by RT-LAMP.

引用

页码：407 / 409

页数：3

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