Genome-Wide Characterization of NBS-Encoding Genes in Watermelon and Their Potential Association with Gummy Stem Blight Resistance

被引:22
|
作者
Hassan, Md Zahid [1 ]
Rahim, Md Abdur [1 ]
Jung, Hee-Jeong [1 ]
Park, Jong-In [1 ]
Kim, Hoy-Taek [1 ]
Nou, Ill-Sup [1 ]
机构
[1] Sunchon Natl Univ, Dept Hort, Sunchon 57922, South Korea
来源
关键词
gummy stem blight; watermelon; resistance; NBS genes; expression; DISEASE RESISTANCE; LRR GENES; VIRUS-RESISTANCE; MELON; IDENTIFICATION; EXPRESSION; PATTERN; PROTEIN; L;
D O I
10.3390/ijms20040902
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons.
引用
收藏
页数:15
相关论文
共 50 条
  • [41] Genome-wide analysis of NBS-encoding resistance genes in the Mediterranean olive tree (Olea europaea subsp. europaea var. europaea): insights into their molecular diversity, evolution and function
    Inchirah Bettaieb
    Dhia Bouktila
    Tree Genetics & Genomes, 2020, 16
  • [42] Genome-Wide Association Analysis of the Genetic Basis for Sheath Blight Resistance in Rice
    Fan Zhang
    Dan Zeng
    Cong-Shun Zhang
    Jia-Ling Lu
    Teng-Jun Chen
    Jun-Ping Xie
    Yong-Li Zhou
    Rice, 2019, 12
  • [43] Genome-Wide Association Analysis of the Genetic Basis for Sheath Blight Resistance in Rice
    Zhang, Fan
    Zeng, Dan
    Zhang, Cong-Shun
    Lu, Jia-Ling
    Chen, Teng-Jun
    Xie, Jun-Ping
    Zhou, Yong-Li
    RICE, 2019, 12 (01)
  • [44] Genome-wide identification and comparative analysis of NBS-LRR resistance genes in Brassica napus
    Alamery, Salman
    Tirnaz, Soodeh
    Bayer, Philipp
    Tollenaere, Reece
    Chaloub, Boulos
    Edwards, David
    Batley, Jacqueline
    CROP & PASTURE SCIENCE, 2018, 69 (01): : 79 - 93
  • [45] Genetic characterization and genome-wide association mapping for stem rust resistance in spring bread wheat
    Shewabez, Elias
    Bekele, Endashaw
    Alemu, Admas
    Mugnai, Laura
    Tadesse, Wuletaw
    BMC GENOMIC DATA, 2022, 23 (01):
  • [46] Genetic characterization and genome-wide association mapping for stem rust resistance in spring bread wheat
    Elias Shewabez
    Endashaw Bekele
    Admas Alemu
    Laura Mugnai
    Wuletaw Tadesse
    BMC Genomic Data, 23
  • [47] Genome-wide association mapping for resistance to bacterial blight and bacterial leaf streak in rice
    Jiang, Nan
    Fu, Jun
    Zeng, Qin
    Liang, Yi
    Shi, Yanlong
    Li, Zhouwei
    Xiao, Youlun
    He, Zhizhou
    Wu, Yuntian
    Long, Yu
    Wang, Kai
    Yang, Yuanzhu
    Liu, Xionglun
    Peng, Junhua
    PLANTA, 2021, 253 (05)
  • [48] Genome-wide nested association mapping of quantitative resistance to northern leaf blight in maize
    Poland, Jesse A.
    Bradbury, Peter J.
    Buckler, Edward S.
    Nelson, Rebecca J.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (17) : 6893 - 6898
  • [49] Genome-wide association mapping for resistance to bacterial blight and bacterial leaf streak in rice
    Nan Jiang
    Jun Fu
    Qin Zeng
    Yi Liang
    Yanlong Shi
    Zhouwei Li
    Youlun Xiao
    Zhizhou He
    Yuntian Wu
    Yu Long
    Kai Wang
    Yuanzhu Yang
    Xionglun Liu
    Junhua Peng
    Planta, 2021, 253
  • [50] Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis (vol 15, pg 809, 2003)
    Meyers, BC
    Kozik, A
    Griego, A
    Kuang, HH
    Michelmore, RW
    PLANT CELL, 2003, 15 (07): : 1683 - 1683