Watermelon (Citrullus lanatus) is a nutritionally rich and economically important horticultural crop of the Cucurbitaceae family. Gummy stem blight (GSB) is a major disease of watermelon, which is caused by the fungus Didymella bryoniae, and results in substantial economic losses in terms of yield and quality. However, only a few molecular studies have focused on GSB resistance in watermelon. Nucleotide binding site (NBS)-encoding resistance (R) genes play important roles in plant defense responses to several pathogens, but little is known about the role of NBS-encoding genes in disease resistance in watermelon. The analyzed NBS-encoding R genes comprises several domains, including Toll/interleukin-1 receptor(TIR), NBS, leucine-rich repeat (LRR), resistance to powdery mildew8(RPW8) and coiled coil (CC), which are known to be involved in disease resistance. We determined the expression patterns of these R genes in resistant and susceptible watermelon lines at different time points after D. bryoniae infection by quantitative RT-PCR. The R genes exhibited various expression patterns in the resistant watermelon compared to the susceptible watermelon. Only six R genes exhibited consistent expression patterns (Cla001821, Cla019863, Cla020705, Cla012430, Cla012433 and Cla012439), which were higher in the resistant line compared to the susceptible line. Our study provides fundamental insights into the NBS-LRR gene family in watermelon in response to D. bryoniae infection. Further functional studies of these six candidate resistance genes should help to advance breeding programs aimed at improving disease resistance in watermelons.
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Habachi-Houimli, Yosra
Khalfallah, Yosra
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Khalfallah, Yosra
Mezghani-Khemakhem, Maha
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Mezghani-Khemakhem, Maha
Makni, Hanem
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Univ Tunis, ISAJC, Bir El Bey, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Makni, Hanem
Makni, Mohamed
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Makni, Mohamed
Bouktila, Dhia
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Univ Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
Univ Jendouba, ISBB, Beja 9000, TunisiaUniv Tunis El Manar, Fac Sci Tunis, Unite Rech Genom Insectes Ravageurs Cultures Inte, GIRC,UR11ES10, Tunis 2092, Tunisia
机构:
Rural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South KoreaRural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South Korea
Mun, Jeong-Hwan
Yu, Hee-Ju
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Rural Dev Adm, Natl Inst Hort & Herbal Sci, Vegetable Res Div, Suwon 441440, South KoreaRural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South Korea
Yu, Hee-Ju
Park, Soomin
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Rural Dev Adm, Natl Inst Hort & Herbal Sci, Vegetable Res Div, Suwon 441440, South KoreaRural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South Korea
Park, Soomin
Park, Beom-Seok
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Rural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South KoreaRural Dev Adm, Natl Acad Agr Sci, Genom Div, Dept Agr Biotechnol, Suwon 441707, South Korea
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Shaoyun Dong
Yanxia Shi
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Yanxia Shi
Zhuonan Dai
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Zhuonan Dai
Han Miao
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Han Miao
Baoju Li
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Baoju Li
Xingfang Gu
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences
Xingfang Gu
Shengping Zhang
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Institute of Vegetables and Flowers,Chinese Academy of Agricultural SciencesInstitute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences