Sex prediction by geometric morphometric analysis of the hard palate

OBJECTIVE: The hard palate participates in the construction of the oral and nasal cavities and represents the bony barrier be-tween them. Morphological differences of the hard palate are important for forensic medicine, anthropology, anatomy, as well as scientific branches dealing with the study of evolutionary development, populations differences. The aim of this study was to determine sexual dimorphism of hard palate on three-dimension-al (3D) models of human skulls using geometric morphometry. MATERIALS AND METHODS: The research was conducted on 3D models of 209 human skulls from Bosnian population (139 male, 70 female). On the obtained 3D models, we marked landmarks on the palate using Landmark editor program. Using MorphoJ program we analyzed sex differences of shape and size on hard palate. RESULTS: The principal component analysis showed that the first two components (PC1 and PC2) described 55.503% of the total morphological variability of the hard palate. The results of the discriminant analysis showed predictive power for male with 66.91% accuracy and for female with 58.57% accuracy based on the shape and size of the hard palate. The influence of size of the hard palate on its shape was statistically significant (p < 0.0001). The results of discriminant analysis based on shape of hard palate showed predictive power for male with 68.34% accuracy and for female with 64.29% accuracy. CONCLUSIONS: Sex differences of hard pal-ate are statistically significant and can be used for sex determination in skeletal remains. The percentage of accuracy for determining sex based on the hard palate was higher for men in this study.