Transcriptome profile of lung dendritic cells after in vitro porcine reproductive and respiratory syndrome virus (PRRSV) infection

被引:17
|
作者
Proell, Maren Julia [1 ]
Neuhoff, Christiane [1 ]
Schellander, Karl [1 ]
Uddin, Muhammad Jasim [2 ]
Cinar, Mehmet Ulas [3 ]
Sahadevan, Sudeep [4 ]
Qu, Xueqi [5 ]
Islam, Md. Aminul [1 ]
Poirier, Mikhael [1 ]
Mueller, Marcel A. [6 ]
Drosten, Christian [6 ]
Tesfaye, Dawit [1 ]
Tholen, Ernst [1 ]
Grosse-Brinkhaus, Christine [1 ]
机构
[1] Univ Bonn, Inst Anim Sci, Dept Anim Breeding & Husb, Bonn, Germany
[2] Univ Queensland, Sch Vet Sci, Gatton Campus, Gatton, Australia
[3] Erciyes Univ, Dept Anim Sci, Fac Agr, Kayseri, Turkey
[4] Univ Copenhagen, BRIC, Copenhagen, Denmark
[5] Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Lab Gene & Cell Engn, Shenzhen, Peoples R China
[6] Charite, Inst Virol, Helmut Ruska Haus, Berlin, Germany
来源
PLOS ONE | 2017年 / 12卷 / 11期
关键词
INNATE IMMUNE-RESPONSE; ALVEOLAR MACROPHAGES; MOLECULAR CHARACTERIZATION; ACTIVATION; DISEASE;
D O I
10.1371/journal.pone.0187735
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The porcine reproductive and respiratory syndrome (PRRS) is an infectious disease that leads to high financial and production losses in the global swine industry. The pathogenesis of this disease is dependent on a multitude of factors, and its control remains problematic. The immune system generally defends against infectious diseases, especially dendritic cells (DCs), which play a crucial role in the activation of the immune response after viral infections. However, the understanding of the immune response and the genetic impact on the immune response to PRRS virus (PRRSV) remains incomplete. In light of this, we investigated the regulation of the host immune response to PRRSV in porcine lung DCs using RNA-sequencing (RNA-Seq). Lung DCs from two different pig breeds (Pietrain and Duroc) were collected before (0 hours) and during various periods of infection (3, 6, 9, 12, and 24 hours post infection (hpi)). RNA-Seq analysis revealed a total of 20,396 predicted porcine genes, which included breed-specific differentially expressed immune genes. Pietrain and Duroc infected lung DCs showed opposite gene expression courses during the first time points post infection. Duroc lung DCs reacted more strongly and distinctly than Pietrain lung DCs during these periods (3, 6, 9, 12 hpi). Additionally, cluster analysis revealed time-dependent co-expressed groups of genes that were involved in immune-relevant pathways. Key clusters and pathways were identified, which help to explain the biological and functional background of lung DCs post PRRSV infection and suggest IL-1 beta 1 as an important candidate gene. RNA-Seq was also used to characterize the viral replication of PRRSV for each breed. PRRSV was able to infect and to replicate differently in lung DCs between the two mentioned breeds. These results could be useful in investigations on immunity traits in pig breeding and enhancing the health of pigs.
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页数:20
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