The recent importation of Grapevine Pinot gris virus into Australia

A total of 575 commercial grapevine (Vitis vinifera) samples from Australia have been tested for a newly emerging virus, Grapevine Pinot gris virus (Trichovirus, Betaflexiviridae) during 2015-2017. Nine samples from two states tested positive. Six of these were from New South Wales and 3 from South Australia in a total of eight varieties. All these varieties were imported from Europe within the last 4-19 years. A fragment of 431 nucleotides on the coat protein gene of Grapevine Pinot gris virus was targeted for virus identification by RT-PCR. The virus specificity of each positive sample was confirmed by sequencing followed by the BLASTn analysis which showed an identity of up to 99.3% to the virus sequences in the NCBI database. The phylogenetic tree as well as pairwise sequence identity showed that, although the virus sequence in each variety was unique, they all grouped with the isolates from Europe and well away from the South Korean and several Chinese isolates. This is the first report of the occurrence of Grapevine Pinot gris virus in Australia.