Cell surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography

被引:53
|
作者
Liu, Jun
McBride, Mark J.
Subramaniam, Sriram [1 ]
机构
[1] NCI, NIH, Canc Res Ctr, Cell Biol Lab, Bethesda, MD 20892 USA
[2] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA
关键词
D O I
10.1128/JB.00957-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Flavobacterium johnsoniae cells glide rapidly over surfaces by an as-yet-unknown mechanism. Using cryoelectron tomography, we show that wild-type cells display tufts of -5-nm-wide cell surface filaments that appear to be anchored to the inner surface of the outer membrane. These filaments are absent in cells of a nonmotile gldF mutant but are restored upon expression of plasmid-encoded GldF, a component of a putative ATP-binding cassette transporter.
引用
收藏
页码:7503 / 7506
页数:4
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