Molecular markers for genic male sterility in Chinese cabbage

被引：36
|
作者：
Ying, M
Dreyer, F
Cai, DG
Jung, C
机构：
[1] Univ Kiel, Inst Crop Sci & Plant Breeding, D-24098 Kiel, Germany
[2] Xiamen Univ, Inst Plant Gene & Gene Technol, Xiamen 361005, Peoples R China
来源：
EUPHYTICA | 2003年 / 132卷 / 02期
关键词：
AFLP; Brassica campestris ssp chinensis; bulked segregant analysis; STS marker;
D O I：
10.1023/A:1024642717416
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The gms gene of Chinese cabbage ( Brassica campestris ssp. chinensis) conferring a recessive genic male sterility was mapped with AFLP markers. Four markers were found to be tightly linked to the gene with a distance of < 1 cM using a mapping population of 327 individuals. The AFLPs were cloned and sequenced. Sequence derived primers yielded clearly distinguishable PCR fragments representing sequences tightly linked to the gms gene. The sequence tagged site (STS) markers can be used for marker assisted selection of male sterile plants among segregating populations.
引用
收藏
页码：227 / 234
页数：8
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