Novel Anti-Tn Single-Chain Fv-Fc Fusion Proteins Derived from Immunized Phage Library and Antibody Fc Domain

被引：0
|
作者：
Kubota, Tsuguo [1 ]
Matsushita, Takefumi [1 ]
Niwa, Rinpei [1 ]
Kumagai, Izumi [2 ]
Nakamura, Kazuyasu [1 ]
机构：
[1] Kyowa Hakko Kirin Co Ltd, Antibody Res Labs, Div Res, Machida, Tokyo 1948533, Japan
[2] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan
来源：
ANTICANCER RESEARCH | 2010年 / 30卷 / 09期
关键词：
Tn antigen; GalNAc(alpha 1-3)-Ser/Thr; single chain antibody; phage-display; Fc fusion; antibody-dependent cellular cytotoxicity (ADCC); DEPENDENT CELLULAR CYTOTOXICITY; MONOCLONAL-ANTIBODIES; HUMAN IGG1; DISPLAY SELECTION; GAMMA-RIII; IN-VITRO; CANCER; CELLS; EXPRESSION; FRAGMENTS;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Tn[GalNAc(alpha 1-3)-Ser/Thr] antigen, a tumor-associated carbohydrate antigen, is highly expressed in various tumors and an attractive candidate for cancer immunotherapy. The generation of an anti-Tn antibody is a first step toward the construction of new anticancer molecules. However, because of the simple and small conformation of the Tn molecule, it is difficult to generate an anti-Tn antibody for therapeutic use by conventional hybridoma technology. The purpose of this study was to isolate anti-Tn single-chain antibody fragments (scFv) by phage display technology from a novel immunised library, to attach an antibody constant region (Fc) and to convert them to scFv-Fc fusion proteins. The scFv-Fcs obtained here showed strict specificity against the Tn antigen and also showed antibody-dependent cellular cytotoxicity. These results suggest a potential use of this antibody generating method by phage display and indicate the potential of Fc-fusion proteins as therapeutic candidates.
引用
收藏
页码：3397 / 3405
页数：9
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