Genome-wide analysis of V-ATPase genes in Plutella xylostella (L.) and the potential role of PxVHA-G1 in resistance to Bacillus thuringiensis Cry1Ac toxin

被引:6
|
作者
Xie, Chao
Xiong, Lei
Ye, Min
Shen, Lingling
Li, Jingge
Zhang, Zhen
You, Minsheng
You, Shijun [1 ]
机构
[1] Fujian Agr & Forestry Univ, Inst Appl Ecol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China
基金
中国国家自然科学基金;
关键词
V-ATPase; Plutella xylostella; Cry1Ac resistance; Bacillus thuringiensis; DIAMONDBACK MOTH; PROTEOMIC ANALYSIS; RNA INTERFERENCE; BINDING-PROTEINS; AMINOPEPTIDASE-N; IDENTIFICATION; MANAGEMENT; MECHANISM; MIDGUT; INSIGHTS;
D O I
10.1016/j.ijbiomac.2021.11.169
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rapid development of insecticide resistance has hampered the use of Bacillus thuringiensis (Bt), a widely used bio-pesticide. Plutella xylostella (L.) is a globally distributed lepidopteran pest of cruciferous vegetables and has developed severe field resistance to the Bt toxin. Vacuolar H+-ATPases (VHA) are multi-subunit complexes and participate in multiple physiological processes. However, the characterization and functional studies of VHA genes are lacking in insects. This study performed a genome-wide analysis and identified 35 VHA gene family members divided into 15 subfamilies in P. xylostella. We cloned a V-ATPase subunit G gene, PxVHA-G1, in our previous midgut transcriptome profiles. Quantitative reverse transcriptase-polymerase chain reaction results showed that PxVHA-G1 was upregulated in the Cry1S1000-resistant strain than in the G88-susceptible strain, and its expression profile revealed that the midgut, Malpighian tubules, and larva stages generally showed high expression levels. RNAi-mediated knockdown of the PxVHA-G1 gene increased the susceptibility of P. xylostella (G88 and Cry1S1000) to Cry1Ac toxin. Our study is the first to explore the role of PxVHA-G1 on regulating Cry1Ac toxicity in P. xylostella, thus, providing new insights into the role of VHAs in the development of Cry1Ac resistance and sustainable development of pest management.
引用
收藏
页码:74 / 83
页数:10
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