Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis

被引：0
|
作者：
Otim, O [1 ]
Singhal, RP [1 ]
机构：
[1] Wichita State Univ, Dept Chem, Wichita, KS 67260 USA
来源：
JOURNAL OF CAPILLARY ELECTROPHORESIS | 1997年 / 4卷 / 05期
关键词：
selectivity; separation; DNA fragments; capillary electrophoresis; gel electrophoresis;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair [Delta bp/Ave.-bp]) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.
引用
收藏
页码：247 / 251
页数：5
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