Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis

被引：0

作者：

Otim, O ^{[1
]}

Singhal, RP ^{[1
]}

机构：

[1] Wichita State Univ, Dept Chem, Wichita, KS 67260 USA

来源：

JOURNAL OF CAPILLARY ELECTROPHORESIS | 1997年 / 4卷 / 05期

关键词：

selectivity; separation; DNA fragments; capillary electrophoresis; gel electrophoresis;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair [Delta bp/Ave.-bp]) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.

引用

页码：247 / 251

页数：5

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