De novo transcriptome assembly and annotation of the third stage larvae of the zoonotic parasite Anisakis pegreffii

被引:10
|
作者
Palomba, Marialetizia [1 ]
Libro, Pietro [1 ]
Di Martino, Jessica [1 ]
Rughetti, Aurelia [2 ]
Santoro, Mario [3 ]
Mattiucci, Simonetta [4 ,5 ]
Castrignano, Tiziana [1 ]
机构
[1] Tuscia Univ, Dept Ecol & Biol Sci, Viale Univ S-N, I-01100 Viterbo, Italy
[2] Sapienza Univ Rome, Dept Expt Med, Ple Aldo Moro 5, I-00185 Rome, Italy
[3] Stn Zool Anton Dohrn, Dept Integrat Marine Ecol, Villa Comunale 1, I-80121 Naples, Italy
[4] Sapienza Univ Rome, Sect Parasitol, Dept Publ Hlth & Infect Dis, Ple Aldo Moro 5, I-00185 Rome, Italy
[5] Fdn Cenci Bolognetti, Ist Pasteur Italia, Rome, Italy
关键词
Anisakis pegreffii; Zoonotic parasite; Transcriptome; De novo assembly; Gene annotation;
D O I
10.1186/s13104-022-06099-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Objectives Anisakis pegreffii is a zoonotic parasite requiring marine organisms to complete its life-history. Human infection (anisakiasis) occurs when the third stage larvae (L3) are accidentally ingested with raw or undercooked infected fish or squids. A new de novo transcriptome of A. pegreffii was here generated aiming to provide a robust bulk of data to be used for a comprehensive "ready-to-use" resource for detecting functional studies on genes and gene products of A. pegreffii involved in the molecular mechanisms of parasite-host interaction. Data description A RNA-seq library of A. pegreffii L3 was here newly generated by using Illumina TruSeq platform. It was combined with other five RNA-seq datasets previously gathered from L3 of the same species stored in SRA of NCBI. The final dataset was analyzed by launching three assembler programs and two validation tools. The use of a robust pipeline produced a high-confidence protein-coding transcriptome of A. pegreffii. These data represent a more robust and complete transcriptome of this species with respect to the actually existing resources. This is of importance for understanding the involved adaptive and immunomodulatory genes implicated in the "cross talk" between the parasite and its hosts, including the accidental one (humans).
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页数:4
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