Transcriptional regulation of CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression pattern in colorectal cancer HCT116 cells

被引：6
|
作者：
Kadziolka, Beata [1 ]
Debski, Konrad J. [1 ]
Bieganowski, Pawel [2 ]
Lesniak, Wieslawa [1 ]
Filipek, Anna [1 ]
机构：
[1] Polish Acad Sci, Nencki Inst Expt Biol, Dept Mol & Cellular Neurobiol, Warsaw, Poland
[2] Polish Acad Sci, Dept Expt Pharmacol, Mossakowski Med Res Ctr, Warsaw, Poland
来源：
IUBMB LIFE | 2018年 / 70卷 / 01期
关键词：
CacyBP; SIP; CREB; E2F1; EGR1; gene expression; microarrays; E2F FAMILY-MEMBERS; NEUROBLASTOMA NB2A; EMERGING ROLES; PROTEIN; RESPONSES; PATHWAY; TARGET; GROWTH; CREB; PROLIFERATION;
D O I：
10.1002/iub.1698
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The CacyBP/SIP protein is expressed at a particularly high level in brain, spleen, and various tumors. In this work, we have studied transcriptional regulation of the CacyBP/SIP gene and the influence of increased CacyBP/SIP level on gene expression in colorectal cancer HCT116 cells. We have shown that E2F1, EGR1, and CREB transcription factors bind to the CacyBP/SIP gene promoter and stimulate transcription of CacyBP/SIP gene. The role of CREB was further confirmed by the observation that forskolin, a strong activator of CREB phosphorylation/activity, increased CacyBP/SIP gene promoter activity. Moreover, we have shown that CREB dominant negative mutants, CREB133 and KCREB, inhibits CacyBP/SIP promoter activity. To check the biological significance of increased CacyBP/SIP expression/level we have applied RNA microarray analysis and have found that upregulation of CacyBP/SIP entails changes in mRNA level of many genes involved, among others, in immune processes. (c) 2017 IUBMB Life, 70(1):50-59, 2018
引用
收藏
页码：50 / 59
页数：10
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