Contributions of TRPV1, endovanilloids, and endoplasmic reticulum stress in lung cell death in vitro and lung injury

被引:35
|
作者
Thomas, Karen C. [1 ]
Roberts, Jessica K. [1 ]
Deering-Rice, Cassandra E. [1 ]
Romero, Erin G. [1 ]
Dull, Randal O. [2 ]
Lee, Jeewoo [3 ]
Yost, Garold S. [1 ]
Reilly, Christopher A. [1 ]
机构
[1] Univ Utah, Dept Pharmacol & Toxicol, Salt Lake City, UT 84112 USA
[2] Univ Utah, Sch Med, Dept Anesthesiol, Salt Lake City, UT 84112 USA
[3] Seoul Natl Univ, Coll Pharm, Seoul, South Korea
基金
新加坡国家研究基金会; 美国国家卫生研究院;
关键词
capsaicin; lipopolysaccharides; lung injury; endovanilloids; transient receptor potential vanilloid-1; INDUCED AIRWAY INFLAMMATION; HYDROGEN-SULFIDE; SUBSTANCE-P; VANILLOID RECEPTOR; ACUTE-PANCREATITIS; CECAL LIGATION; GENE-PRODUCTS; ORGAN INJURY; ACTIVATION; SEPSIS;
D O I
10.1152/ajplung.00231.2011
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Thomas KC, Roberts JK, Deering-Rice CE, Romero EG, Dull RO, Lee J, Yost GS, Reilly CA. Contributions of TRPV1, endovanilloids, and endoplasmic reticulum stress in lung cell death in vitro and lung injury. Am J Physiol Lung Cell Mol Physiol 302: L111-L119, 2012. First published September 23, 2011; doi: 10.1152/ajplung.00231.2011.-Endogenous agonists of transient receptor potential vanilloid-1 (TRPV1) (endovanilloids) are implicated as mediators of lung injury during inflammation. This study tested the hypothesis that endovanilloids produced following lipopolysaccharide (LPS) treatment activate TRPV1 and cause endoplasmic reticulum stress/GADD153 expression in lung cells, representing a mechanistic component of lung injury. The TRPV1 agonist nonivamide induced GADD153 expression and caused cytotoxicity in immortalized and primary human bronchial, bronchiolar/alveolar, and microvascular endothelial cells, proportional to TRPV1 mRNA expression. In CF-1 mice, Trpv1 mRNA was most abundant in the alveoli, and intratracheal nonivamide treatment promoted Gadd153 expression in the alveolar region. Treatment of CF-1 mice with LPS increased Gadd153 in the lung, lactate dehydrogenase (LDH) in bronchoalveolar lavage (BAL) fluid, and lung wet-to-dry weight ratio. Cotreating mice with LPS and the TRPV1 antagonist LJO-328 reduced Gadd153 induction and LDH in BAL but did not inhibit increases in lung wet-to-dry ratio. In Trpv1(-/-) mice treated with LPS, Gadd153 induction and LDH in BAL were reduced relative to wild-type mice, and the wet-to-dry weight ratios of lungs from both wild-type and Trpv1(-/-) mice decreased. Organic extracts of blood collected from LPS-treated mice were more cytotoxic to TRPV1-overexpressing cells compared with BEAS-2B cells and extracts from control mice, however, most pure endovanilloids did not produce cytotoxicity in a characteristic TRPV1-dependent manner. Collectively, these data indicate a role for TRPV1, and endogenous TRPV1 agonists, in ER stress and cytotoxicity in lung cells but demonstrate that ER stress and cytotoxicity are not essential for pulmonary edema.
引用
收藏
页码:L111 / L119
页数:9
相关论文
共 50 条
  • [41] Anti-inflammatory effects of Esomeprazole in septic lung injury by mediating endoplasmic reticulum stress
    Wang, Peng
    Li, Hui
    Wu, Wencheng
    JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2025,
  • [42] Dexpanthenol ameliorates doxorubicin-induced lung injury by regulating endoplasmic reticulum stress and apoptosis
    Muhammet Yusuf Tepebaşı
    Halil İbrahim Büyükbayram
    Özlem Özmen
    Şerife Taşan
    Esma Selçuk
    Naunyn-Schmiedeberg's Archives of Pharmacology, 2023, 396 : 1837 - 1845
  • [43] Protein Misfolding and Endoplasmic Reticulum Stress in Chronic Lung Disease
    Wei, James
    Rahman, Sadaf
    Ayaub, Ehab A.
    Dickhout, Jeffrey G.
    Ask, Kjetil
    CHEST, 2013, 143 (04) : 1098 - 1105
  • [44] Involvement of Endoplasmic Reticulum Stress in Myofibroblastic Differentiation of Lung Fibroblasts
    Baek, Hyun Ah
    Kim, Do Sung
    Park, Ho Sung
    Jang, Kyu Yun
    Kang, Myoung Jae
    Lee, Dong Geun
    Moon, Woo Sung
    Chae, Han Jung
    Chung, Myoung Ja
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2012, 46 (06) : 731 - 739
  • [45] A new target for caffeine in the developing lung: endoplasmic reticulum stress?
    Rath, Philipp
    Nardiello, Claudio
    Morty, Rory E.
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2017, 313 (04) : L659 - L663
  • [46] Endoplasmic reticulum stress and glutathione therapeutics in chronic lung diseases
    Janssen-Heininger, Yvonne
    Reynaert, Niki L.
    van der Vliet, Albert
    Anathy, Vikas
    REDOX BIOLOGY, 2020, 33
  • [47] ENDOPLASMIC RETICULUM STRESS CORRELATES WITH FIBROSIS IN INTERSTITIAL LUNG DISEASE
    Parfrey, H.
    Moseley, E.
    Beardsley, B.
    Knight, J.
    Marciniak, S. J.
    Rassl, D.
    THORAX, 2016, 71 : A44 - A45
  • [48] Quantitative Assessment of TRPV1 in Lung Transplant Recipients.
    Duarte, A. G.
    Campbell, G. A.
    Myers, A. C.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179
  • [49] Diet-Induced Obesity Induces Endoplasmic Reticulum Stress In The Lung Endothelium And Predispose To LPS Induced Acute Lung Injury
    Shah, D.
    Romero, F.
    Summer, R.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2016, 193
  • [50] Role of Endoplasmic Reticulum Stress in Retinal Ganglion Cell Death in Glaucoma and Optic Nerve Injury
    Yang, Z.
    Ge, Y.
    Pease, M.
    Quigley, H.
    Zack, D. J.
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (13)