A mitochondria-targeted single fluorescence probe for separately and continuously visualizing H2S and Cys with multi-response signals

被引:32
|
作者
Zhao, Xinxin [1 ]
Ji, Hefang [1 ]
Hasrat, Kamran [1 ]
Misal, Saima [1 ]
He, Fangru [1 ]
Dai, Yanpeng [1 ]
Ma, Fulong [1 ]
Qi, Zhengjian [1 ]
机构
[1] Southeast Univ, Sch Chem & Chem Engn, Jiangsu Prov Hitech Key Lab Biomed Res, Nanjing 211189, Peoples R China
关键词
GALACTOSIDASE ACTIVITY DETECTION; AGGREGATION-INDUCED EMISSION; LARGE STOKES SHIFT; SELECTIVE DETECTION; HYDROGEN-SULFIDE; RATIONAL DESIGN; QUANTUM DOTS; CELLS; HYPOCHLORITE; METABOLISM;
D O I
10.1016/j.aca.2020.02.017
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Hydrogen sulfide and cysteine are momentous endogenous regulators of many physiological processes and maintain a dynamic balance of redox in living organisms. To investigate the inter-relationship of them in vivo, there is a pressing need to develop analytical molecular tools to identify related biomolecules. We construct a mitochondria-targeted single fluorescence probe (Mit-CM) for separately and continuously visualizing H2S, Cys and H2S/Cys with multi-response fluorescence signals. Mit-CM has the following advantages: (I) colorimetric and ratiometric: two well-separated emission bands can ensure accurate detection of the analyte and significant color changes contribute to rapid identification of the analyte by the naked eye; (II) mitochondrial localization: study the physiological functions of H2S and Cys in mitochondria; (III) separate and continuous detection of H2S and Cys: reveal the inter-relationship and interconversion of them in biological system. Moreover, the desirable attributes of low cytotoxicity, better biocompatibility and excellent mitochondria enrichment ability indicate that Mit-CM can be employed to achieve detection and observe distribution of H2S, Cys and H2S/Cys in living organism. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页码:172 / 182
页数:11
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