Point spread function of optical microscopes imaging through stratified media

被引：50
|
作者：
Haeberlé, O
Ammar, M
Furukawa, H
Tenjimbayashi, K
Török, P
机构：
[1] Univ Haute Alsace, Grp Lab E1, Lab MIPS, F-68093 Mulhouse, France
[2] AIST, OptMec, Photon Res Inst, Tsukuba, Ibaraki 3058564, Japan
[3] Univ London Imperial Coll Sci Technol & Med, Blackett Lab, London SW7 2BW, England
来源：
OPTICS EXPRESS | 2003年 / 11卷 / 22期
关键词：
D O I：
10.1364/OE.11.002964
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We propose a model for imaging point objects through a dielectric interface or stratified media. The model is applicable to conventional and confocal fluorescence microscopy, with single- or multi-photon excitation. An analytical solution is obtained in the form of readily computable functions. When large mismatches occur in the refractive indices of the media of the objective lens and specimen the illumination and detection point spread functions differ significantly, showing that currently used imaging models may fail to correctly predict imaging properties of optical microscopes. (C) 2003 Optical Society of America.
引用
收藏
页码：2964 / 2969
页数：6
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