Zinc-Finger Nucleases: A Panoramic View

被引：32
|
作者：
Carroll, Dana [1 ]
机构：
[1] Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA
来源：
CURRENT GENE THERAPY | 2011年 / 11卷 / 01期
关键词：
DNA repair; gene targeting; homologous recombination; nonhomologous end joining (NHEJ); zinc-finger nuclease (ZFN); zinc fingers; DOUBLE-STRAND BREAKS; TRANSCRIPTION FACTOR-IIIA; TARGETED MUTAGENESIS; BINDING DOMAINS; HOMOLOGOUS RECOMBINATION; RESTRICTION ENZYMES; GENOME MODIFICATION; DNA-RECOGNITION; GENE-THERAPY; SELECTION;
D O I：
10.2174/156652311794520076
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Zinc-finger nucleases (ZFNs) are emerging as very powerful tools for directed genome modifications. Their key features are: a DNA-binding domain comprised of zinc fingers that can be designed to favor very specific targets; a nonspecific cleavage domain that must dimerize to cut DNA - this requirement enhances specificity and minimizes random cleavage. ZFNs have been shown to be effective in a wide range of organisms and cell types. This article reviews discoveries that led to the development of ZFNs, cites examples of successes in genome engineering, and projects how ZFNs may be used in the future, particularly in applications to humans.
引用
收藏
页码：2 / 10
页数：9
相关论文
共 50 条
  • [31] Targeted mutagenesis in zebrafish using customized zinc-finger nucleases
    Foley, Jonathan E.
    Maeder, Morgan L.
    Pearlberg, Joseph
    Joung, J. Keith
    Peterson, Randall T.
    Yeh, Jing-Ruey J.
    NATURE PROTOCOLS, 2009, 4 (12) : 1855 - 1868
  • [32] Progress and prospects: Zinc-finger nucleases as gene therapy agents
    D Carroll
    Gene Therapy, 2008, 15 : 1463 - 1468
  • [33] Zinc-finger nucleases: new strategies to target the rat genome
    Geurts, Aron M.
    Moreno, Carol
    CLINICAL SCIENCE, 2010, 119 (7-8) : 303 - 311
  • [34] Highly active zinc-finger nucleases by extended modular assembly
    Bhakta, Mital S.
    Henry, Isabelle M.
    Ousterout, David G.
    Das, Kumitaa Theva
    Lockwood, Sarah H.
    Meckler, Joshua F.
    Wallen, Mark C.
    Zykovich, Artem
    Yu, Yawei
    Leo, Heather
    Xu, Lifeng
    Gersbach, Charles A.
    Segal, David J.
    GENOME RESEARCH, 2013, 23 (03) : 530 - 538
  • [35] Generation of albino Xenopus tropicalis using zinc-finger nucleases
    Nakajima, Keisuke
    Nakajima, Taeko
    Takase, Minoru
    Yaoita, Yoshio
    DEVELOPMENT GROWTH & DIFFERENTIATION, 2012, 54 (09) : 777 - 784
  • [36] Genetic modification: Targeted manipulation of the genome with zinc-finger nucleases
    Meyer M.
    Wefers B.
    Ortiz O.
    Wurst W.
    Kühn R.
    BIOspektrum, 2011, 17 (5) : 537 - 540
  • [37] Zinc-finger nucleases: a powerful tool for genetic engineering of animals
    Séverine Rémy
    Laurent Tesson
    Séverine Ménoret
    Claire Usal
    Andrew M. Scharenberg
    Ignacio Anegon
    Transgenic Research, 2010, 19 : 363 - 371
  • [38] Targeted mutagenesis in zebrafish using customized zinc-finger nucleases
    Jonathan E Foley
    Morgan L Maeder
    Joseph Pearlberg
    J Keith Joung
    Randall T Peterson
    Jing-Ruey J Yeh
    Nature Protocols, 2009, 4 : 1855 - 1868
  • [39] Progress and prospects: Zinc-finger nucleases as gene therapy agents
    Carroll, D.
    GENE THERAPY, 2008, 15 (22) : 1463 - 1468
  • [40] Zinc-finger nucleases: how to play two good hands
    Mark Isalan
    Nature Methods, 2012, 9 : 32 - 34
12345