Zinc-Finger Nucleases: A Panoramic View

被引：32

作者：

Carroll, Dana ^{[1
]}

机构：

[1] Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA

来源：

CURRENT GENE THERAPY | 2011年 / 11卷 / 01期

关键词：

DNA repair; gene targeting; homologous recombination; nonhomologous end joining (NHEJ); zinc-finger nuclease (ZFN); zinc fingers; DOUBLE-STRAND BREAKS; TRANSCRIPTION FACTOR-IIIA; TARGETED MUTAGENESIS; BINDING DOMAINS; HOMOLOGOUS RECOMBINATION; RESTRICTION ENZYMES; GENOME MODIFICATION; DNA-RECOGNITION; GENE-THERAPY; SELECTION;

D O I：

10.2174/156652311794520076

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Zinc-finger nucleases (ZFNs) are emerging as very powerful tools for directed genome modifications. Their key features are: a DNA-binding domain comprised of zinc fingers that can be designed to favor very specific targets; a nonspecific cleavage domain that must dimerize to cut DNA - this requirement enhances specificity and minimizes random cleavage. ZFNs have been shown to be effective in a wide range of organisms and cell types. This article reviews discoveries that led to the development of ZFNs, cites examples of successes in genome engineering, and projects how ZFNs may be used in the future, particularly in applications to humans.

引用

页码：2 / 10

页数：9

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