Background: College students are at the junction of study and employment. At the same time, they are facing the dual pressure of study and employment, so they are very easy to have negative emotions. Anxiety is an emotional experience that people often perceive in daily life. When individuals encounter external stimuli or face setbacks, they will show a series of negative emotions, such as tension, worry, irritability and so on. Employment anxiety is a comprehensive response to negative emotions such as worry and tension caused by the perceived gap between internal consciousness and objective reality when individuals face employment problems, mainly including self-awareness anxiety, social support anxiety, employment situation anxiety, fear of self-esteem damage, etc. Therefore, the research analyzes students' employment anxiety based on educational psychology, and integrates ecological Marxism theory into innovation and entrepreneurship education, so as to improve the innovation and entrepreneurship education model and alleviate students' employment anxiety. Subjects and methods: In a certain university, 50 senior students who are about to graduate and facing employment are selected for the experiment. Use computer information technology to complete the grouping, and get group A and group B. The number of students in group A and group B is the same. For group A, the research adopts the improved innovation and entrepreneurship education based on educational psychology to teach them. For group B, the traditional innovation and entrepreneurship education was used to teach them. After using different teaching modes for the same time, the two groups of students' anxiety and psychological state about the employment situation were compared. Results: Before teaching, the scores of anxiety subscale, thought subscale and body subscale in EAS of group A and group B students were very close, and there was little difference between the two groups. After the intervention of different teaching modes on students, the subscale scores and total scale scores of group A students have decreased significantly, while the scale scores of group B students have not changed significantly. Conclusions: For various reasons, college students are easy to suffer from employment anxiety. Innovation and entrepreneurship education is a course to cultivate students' entrepreneurial consciousness and innovative spirit. It is an important way to alleviate students' employment anxiety. However; today's curriculum rarely involves the contents of environment and ecology, which is contrary to the green development and sustainable development advocated by our country and divorced from reality. The research analyzes students' employment anxiety based on educational psychology, and integrates ecological Marxism theory into innovation and entrepreneurship education, so as to improve the innovation and entrepreneurship education model and alleviate students' employment anxiety.