A quantum dot-based lateral flow immunoassay for the sensitive detection of human heart fatty acid binding protein (hFABP) in human serum

被引：42
|
作者：
Savin, Mihaela [1 ,2 ]
Mihailescu, Carmen-Marinela [3 ]
Matei, Iulia [4 ,5 ]
Stan, Dana [2 ]
Moldovan, Carmen Aura [6 ]
Ion, Marian [6 ]
Baciu, Ion [1 ]
机构：
[1] Univ Bucharest, Fac Chem, Regina Elisabeta Bd 4-12, Bucharest, Romania
[2] Telemedica, Jean Louis Calderonno 28, Bucharest, Romania
[3] DDS Diagnost, Vatra Luminoasa 45, Bucharest 031563, Romania
[4] Romanian Acad, Ilie Murgulescu Inst Phys Chem, 202 Splaiul Independentei, Bucharest 060021, Romania
[5] Univ Bucharest, Fac Chem, Dept Phys Chem, Bd Regina Elisabeta 4-12, Bucharest, Romania
[6] Natl Inst R&D Microtechnol IMT Bucharest, 126 A,Erou Iancu Nicolae St, Bucharest, Romania
来源：
TALANTA | 2018年 / 178卷
关键词：
Lateral flow immuno assay; H-FABP; Acute myocardial infarction; Quantum dots; Fluorescence detection; ACUTE MYOCARDIAL-INFARCTION; H-FABP; CDTE; BIOSENSOR;
D O I：
10.1016/j.talanta.2017.10.045
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We describe the preparation and validation of a novel lateral flow immunoassay test for the detection of human heart fatty acid binding protein (hFABP). Water-soluble CdTe quantum dots (QDs) were selected as the fluorescent label and were linked covalently to anti-hFABP antibodies. Upon conjugation, the secondary structure of the anti-hFABP was preserved and the fluorescence quantum yield of the CdTe QDs increased. The labelled antibodies were transferred to the immunoassay test strip and the antigen-antibody reaction was successfully performed. This evidenced the preserved antibody activity of QD-labelled anti-hFABP towards hFABP, and provided a rapid means for the quantitation of hFABP in human serum within the range of 0-160 ng . ml(-1), with a much lower detection limit of 221 pg.ml(-1) compared with other rapid tests based on lateral flow immunoassays. This new immunoassay test has been successfully used for the early detection of acute myocardial infarction.
引用
收藏
页码：910 / 915
页数：6
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