Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes

被引：10

作者：

Chang, Yung-Fu ^{[2
]}

Chou, Hui-Ju ^{[2
]}

Yen, Ying-Cheng ^{[2
]}

Chang, Hsueh-Wei ^{[2
]}

Hong, Yi-Ren ^{[4
]}

Huang, Hurng-Wern ^{[1
,5
]}

Tseng, Chao-Neng ^{[1
,2
,3
]}

机构：

[1] Kaohsiung Med Univ, Grad Inst Nat Prod, Kaohsiung 807, Taiwan

[2] Kaohsiung Med Univ, Dept Biomed Sci & Environm Biol, Kaohsiung 807, Taiwan

[3] Kaohsiung Med Univ, Ctr Canc, Kaohsiung Med Univ Hosp, Kaohsiung 807, Taiwan

[4] Kaohsiung Med Univ, Grad Inst Biochem, Kaohsiung 807, Taiwan

[5] Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung 804, Taiwan

来源：

FEBS LETTERS | 2012年 / 586卷 / 19期

关键词：

Neuromuscular junction; mRNA localization; mRNA targeting; Synapse; AChR; Chrna1; Agrin; Staufen; NEUROMUSCULAR-JUNCTION; PROTEIN-SYNTHESIS; SKELETAL-MUSCLE; GENE-EXPRESSION; TRANSLATIONAL CONTROL; SYNAPTIC SITES; DENDRITES; LOCALIZATION; STAUFEN1; NEURONS;

D O I：

10.1016/j.febslet.2012.07.068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the mammalian central nervous system transcripts of certain synaptic components are localized near the synapse, allowing for rapid regulation of protein levels. Here we test whether an mRNA localization mechanism also exists in the postsynaptic specialization induced by agrin in C2C12 myotubes. RT-PCR showed that Chrna1 was co-purified with nicotinic acetylcholine receptor (AChR) isolated by affinity column or by ultracentrifugation. In addition, Stau1 was found to interact with Chrna1 mRNA, and knocking down of Stau1 by RNAi resulted in defective AChR clustering. These results suggest that mRNA localization also participates in the formation of mammalian neuromuscular junction (NMJ). Structured summary of protein interactions: AChR alpha 1 physically interacts with Stau1 by pull down (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3111 / 3116

页数：6

共 50 条

[31] Interaction of integrin α7β1 in C2C12 myotubes and in solution with laminin
Zolkiewska, A
Thompson, WC
Moss, J
EXPERIMENTAL CELL RESEARCH, 1998, 240 (01) : 86 - 94
[32] A dinucleotide repeat and a Hae III polymorphism in the human nicotinic acetylcholine receptor alpha-subunit gene (CHRNA1)
Djabiri, F
Gomez, L
Bach, JF
Garchon, HJ
CLINICAL GENETICS, 1997, 52 (02) : 130 - 132
[33] Heat Acclimation Mediated Crosstolerance In C2C12 Myotubes
Hill, Garrett W.
Lee, Ben J.
Gillum, Trevor L.
Vaughan, Roger A.
Kuennen, Matthew R.
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 2019, 51 (06): : 467 - 467
[34] Sphingolipid involvement in insulin sensitivity in C2C12 myotubes
Cowart, LA
Hui, W
FASEB JOURNAL, 2006, 20 (05): : A1345 - A1345
[35] Inflammation modulates the activation of autophagy in C2C12 myotubes
Rogers, Robert
Howe, Parker
Hayes, Annemarie
Yeung, Justin
Staudinger, Jeffrey
PHYSIOLOGY, 2023, 38
[36] Heat Treatment Regulates Autophagy in C2C12 Myotubes
Summers, Corey M.
Valentine, Rudy J.
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 2017, 49 (05): : 499 - 499
[37] Effect of nicorandil on insulin sensitivity of C2C12 myotubes
Dymkowska, D.
Szczepanowska, J.
Zablocki, K.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2012, 1817 : S119 - S119
[38] β-Taxilin participates in differentiation of C2C12 myoblasts into myotubes
Sakane, Hiroshi
Makiyama, Tomohiko
Nogami, Satoru
Horii, Yukimi
Akasaki, Kenji
Shirataki, Hiromichi
EXPERIMENTAL CELL RESEARCH, 2016, 345 (02) : 230 - 238
[39] Mechanism of DCEBIO induced hypertrophy of C2C12 myotubes
Sakamoto, Kazuho
Tanaka, Shoko
JOURNAL OF PHARMACOLOGICAL SCIENCES, 2017, 133 (03) : S195 - S195
[40] Ethanol decreases acetylcholine receptor clustering in C2C12 myotube culture
Owen, David B.
grow, Wade A.
FASEB JOURNAL, 2007, 21 (05): : A233 - A233

← 1 2 3 4 5 →