Immobilization of Laccase on Epoxy Group-functionalized Periodic Mesoporous Organosilicas

被引：0
|
作者：
Lan Jia-Ning [1 ]
Na Wei [1 ]
Wei Qi [1 ]
Li Qun-Yan [1 ]
Wang Wei [1 ]
Nie Zuo-Ren [1 ]
机构：
[1] Beijing Univ Technol, Coll Mat Sci & Engn, Beijing 100124, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2010年 / 31卷 / 08期
关键词：
Periodic mesoporous organosilica; Epoxy group; Laccase; Immobilization; Stability; ORGANIC-INORGANIC MATERIALS; PENICILLIN-G ACYLASE; ENZYME IMMOBILIZATION; MOLECULAR-SIEVES; SILICA; SBA-15; STABILITY; CHANNEL; ROUTE;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Epoxy group-functionalized periodic mesoporous organosilicas(FPMOs) was synthesized by the co-condensation of 1,2-bis(triethoxysilyl) ethane(BTESE) and 3-glycidoxypropyltrimethoxylsilane(GPTMS) in the presence of triblock copolymer poly(ethylene glycol)-b-poly(propylene glycol) -b-poly (ethylene glycol) (P123) under acidic conditions and utilized as a support for laccase immobilization. The effect of epoxy group-functionalization on the immobilization of laccase and the stability of immobilized laccase were investigated in detail. The level of functionalization, the pore structure and the laccase immobilization were detected by means of solid-state NMR, N-2 adsorption and UV-Vis, respectively. It is revealed that epoxy groups are successfully attached covalently to the pore wall of PMOs and the functionalized materials retain a desirable pore structure. Laccase immobilized on functionalized FPMOs exhibits a higher activity and stability than that adsorbed on pure PMOs.
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页码：1579 / 1584
页数：6
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