Immobilization of Laccase on Epoxy Group-functionalized Periodic Mesoporous Organosilicas

被引：0
|
作者：
Lan Jia-Ning [1 ]
Na Wei [1 ]
Wei Qi [1 ]
Li Qun-Yan [1 ]
Wang Wei [1 ]
Nie Zuo-Ren [1 ]
机构：
[1] Beijing Univ Technol, Coll Mat Sci & Engn, Beijing 100124, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2010年 / 31卷 / 08期
关键词：
Periodic mesoporous organosilica; Epoxy group; Laccase; Immobilization; Stability; ORGANIC-INORGANIC MATERIALS; PENICILLIN-G ACYLASE; ENZYME IMMOBILIZATION; MOLECULAR-SIEVES; SILICA; SBA-15; STABILITY; CHANNEL; ROUTE;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Epoxy group-functionalized periodic mesoporous organosilicas(FPMOs) was synthesized by the co-condensation of 1,2-bis(triethoxysilyl) ethane(BTESE) and 3-glycidoxypropyltrimethoxylsilane(GPTMS) in the presence of triblock copolymer poly(ethylene glycol)-b-poly(propylene glycol) -b-poly (ethylene glycol) (P123) under acidic conditions and utilized as a support for laccase immobilization. The effect of epoxy group-functionalization on the immobilization of laccase and the stability of immobilized laccase were investigated in detail. The level of functionalization, the pore structure and the laccase immobilization were detected by means of solid-state NMR, N-2 adsorption and UV-Vis, respectively. It is revealed that epoxy groups are successfully attached covalently to the pore wall of PMOs and the functionalized materials retain a desirable pore structure. Laccase immobilized on functionalized FPMOs exhibits a higher activity and stability than that adsorbed on pure PMOs.
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页码：1579 / 1584
页数：6
相关论文
共 28 条
  • [1] ABADULIA F, 2000, APPL ENVIRON MICROB, V66, P33579
  • [2] Periodic mesoporous organosilicas with organic groups inside the channel walls
    Asefa, T
    MacLachlan, MJ
    Coombs, N
    Ozin, GA
    [J]. NATURE, 1999, 402 (6764) : 867 - 871
  • [3] Novel bifunctional periodic mesoporous organosilicas, BPMOs: Synthesis, characterization, properties and in-situ selective hydroboration-alcoholysis reactions of functional groups
    Asefa, T
    Kruk, M
    MacLachlan, MJ
    Coombs, N
    Grondey, H
    Jaroniec, M
    Ozin, GA
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (35) : 8520 - 8530
  • [4] BECK JS, 1992, J AM CHEM SOC, V114, P10843
  • [5] Characterization of glutamate dehydrogenase immobilization on silica surface by atomic force microscopy and kinetic analyses
    Blasi, L
    Longo, L
    Vasapollo, G
    Cingolani, R
    Rinaldi, R
    Rizzello, T
    Acierno, R
    Maffia, M
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (5-6) : 818 - 823
  • [6] Enzyme immobilization in MCM-41 molecular sieve
    Diaz, JF
    Balkus, KJ
    [J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) : 115 - 126
  • [7] Gao B, 2005, CHEM J CHINESE U, V26, P1852
  • [8] Gao B, 2003, CHEM J CHINESE U, V24, P1100
  • [9] Triblock copolymer synthesis of highly ordered large-pore periodic mesoporous organosilicas with the aid of inorganic salts
    Guo, WP
    Park, JY
    Oh, MO
    Jeong, HW
    Cho, WJ
    Kim, I
    Ha, CS
    [J]. CHEMISTRY OF MATERIALS, 2003, 15 (12) : 2295 - +
  • [10] Chloroperoxidase on periodic mesoporous organosilanes: Immobilization and reuse
    Hudson, S.
    Cooney, J.
    Hodnett, B. K.
    Magner, E.
    [J]. CHEMISTRY OF MATERIALS, 2007, 19 (08) : 2049 - 2055
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