Effect of Silver and Titanium Dioxide Nanoparticles on PCR Efficiency

被引:0
|
作者
Wan, Weijie [1 ]
Yeow, John T. W. [1 ]
Van Dyke, Michele I. [2 ]
机构
[1] Univ Waterloo, Dept Syst Design Engn, Waterloo, ON N2L 3G1, Canada
[2] Univ Waterloo, Dept Civil & Environm Engn, Waterloo, ON, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
polymerase chain reaction (PCR); nanomaterials; silver nanoparticles; titanium dioxide nanoparticles; POLYMERASE-CHAIN-REACTION; ENZYMATIC AMPLIFICATION; CELL;
D O I
暂无
中图分类号
TM [电工技术]; TN [电子技术、通信技术];
学科分类号
0808 ; 0809 ;
摘要
Interaction between nanomaterials and biological systems and the role nanomaterials play in biochemical reactions is of great interest nowadays due to the unique properties of nanomaterials and their potential applications in Biomedical Engineering. Recently, nanoparticle PCR research has attracted more attention. However, research has been focused on gold nanoparticles exclusively and there is no work about the effect of other nanoparticles reported yet. In this work, the effect of silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (TiO(2)NPs) is evaluated using a real-time PCR machine. It is found that AgNPs start to cause PCR inhibition at 30 mu g/mL. TiO(2)NPs exhibit much stronger inhibitory effect. They start to cause PCR inhibition at 0.8 mu g/mL. When different amounts of AgNPs and TiO(2)NPs are added to PCR solution, some interesting phenomenon is observed. The effect of the combination of AgNPs and TiO(2)NPs is not simply the linear combination of the effect of individual AgNPs and TiO(2)NPs. However, by choosing the combination of AgNPs and TiO(2)NPs, PCR inhibition can be minimized. The observations suggest that a complex interaction mechanism exists between AgNPs and TiO(2)NPs.
引用
收藏
页码:458 / 461
页数:4
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