Neuroprotective effects of lamotrigine and remacemide on excitotoxicity induced by glutamate agonists in isolated chick retina

The possible neuroprotective effects of two recently developed antiepileptic compounds, lamotrigine (LTG) and remacemide (REMA), against glutamate agonist-induced excitotoxicity have been investigated in the isolated chick embryo retina model. Retina segments from 15- or Is-day-old embryos were incubated in 1 ml of balanced salt solution, at 25 degreesC for 30 min, in the presence or absence of N-methyl-D-aspartate (NMDA), kainic acid (KA), or alpha -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) (10 to 200 muM). LTG, REMA, and the active desglycinyl metabolite of REMA (d-REMA) (10-200 muM) were added separately 5 min before glutamate agonists. Retina damage was assessed after 24 h (i) by measuring LDH activity present in the medium, expressed as percentage of total retina LDH activity, and (ii) by histological analysis of retina specimens through scoring for the presence or absence of edema, necrosis, nuclear pyknosis, and cell layer damage. LTG, REMA, and d-REMA reduced LDH release produced by NMDA 58-70% in a dose-dependent manner, with d-REMA being the most potent (EC50: d-REMA, 25.75 +/-3.27 muM; REMA 64.75 +/-7.75 muM; LTG, 60.50 +/-6.80 muM; P < 0.001). The drugs had less effect on the LDH release produced by AMPA and Kk Histological analysis confirmed these biochemical results, with all three compounds reducing edema and the number of necrotic and pyknotic nuclei in the ganglion layer. d-REMA provided almost complete protection of the ganglion cell layer against damage produced by NMDA. Combinations of d-REMA and LTG showed additive rather than potentiative effects against NMDA-induced cell injury. The present data provide pharmacological evidence that LTG, REMA, and d-REMA decrease glutamate agonist-induced excitotoxicity in isolated chick retina, findings that might have therapeutic implications for various neurological (C) 2001 Academic Press.